CAJ | 학술논문

　　通过考察转ATCIPK23基因烟草植株在不同钾浓度下钾及干物质积累的能力，研究了外源基因的导入对转基因烟草植株钾吸收能力的影响。结果表明，在低钾环境下，转基因株系具有明显的生长优势，烟株发育较快、根系发达、生物量较大、植株的含钾率也有明显提高，随着供钾量的减少，这种差异幅度逐渐增大。当供钾浓度为0．1 mmol?L-1时，对照品种K326植株的平均钾含量为17．7 g?kg-1，而转基因品系KA12为23．3 g?kg-1，KA16为24．6 g?kg-1，KA21为22．1 g?kg-1，分别较对照品种增加了31．6％、39．0％和27．7％。钾吸收动力学研究表明，转基因植株与对照品种植株的最大吸收效率（Vmax ）差别不大，而最低浓度（Cmin ）与米氏常数（KM ）的差别较为明显。
　　통과고찰전ATCIPK23기인연초식주재불동갑농도하갑급간물질적루적능력，연구료외원기인적도입대전기인연초식주갑흡수능력적영향。결과표명，재저갑배경하，전기인주계구유명현적생장우세，연주발육교쾌、근계발체、생물량교대、식주적함갑솔야유명현제고，수착공갑량적감소，저충차이폭도축점증대。당공갑농도위0．1 mmol?L-1시，대조품충K326식주적평균갑함량위17．7 g?kg-1，이전기인품계KA12위23．3 g?kg-1，KA16위24．6 g?kg-1，KA21위22．1 g?kg-1，분별교대조품충증가료31．6％、39．0％화27．7％。갑흡수동역학연구표명，전기인식주여대조품충식주적최대흡수효솔（Vmax ）차별불대，이최저농도（Cmin ）여미씨상수（KM ）적차별교위명현。
The experiment was conducted to study the ability of K+ and dry matter accumulation of ATCIPK23 transgenic tobacco lines under different K+ concentration .The results showed that there were remarkable growth advan-tages for the transgenic tobacco lines ,whose plant growth rate ,root development ,dry matter accumulation and plant potassium content were wholly improved remarkably ,compared with the contrast variety .These effects tended to become stronger along with the reduction of potassium supply .When the K+ concentration of culture medium dropped to 0 .1 mmol?L-1 ,the potassium content in the above-ground part of contrast variety K326 was 17 .7 g?kg-1 ,and that of the transgenic lines KA12 ,KA16 and KA21 was 23 .3 g?kg-1 ,24 .6 g?kg-1 and 22 .1 g?kg-1 respectively ,being in-creased by 31 .6% ,39 .0% and 27 .7% respectively .The study on potassium absorption kinetics indicated that there was no significant difference in maximum absorption efficiency (Vmax ) between the transgenic lines and the contrast vari-ety ,but there were significant differences in minimum concentration (Cmin ) and Michaelis constant (KM ) between them .