CAJ | 학술논문

　　以己唑醇为配体与醋酸铜配合获得元素组成异化的己唑醇铜配合物。配合物对小麦种子浸种处理，待萌发生长至幼苗阶段将其进行NaCl胁迫处理，通过对幼苗脯氨酸含量以及脯氨酸合成酶基因 P5CS、P5CR表达变化的测定，评价己唑醇铜配合物对植物抗盐能力的影响。结果表明：小麦幼苗脯氨酸含量随着盐浓度的增大及胁迫时间的延长而增加，200 mmol?L-1 NaCl胁迫下，配合物处理后脯氨酸含量最高，达55．44μg?g-1，相比己唑醇和醋酸铜处理分别增加13．68％和58．45％；而100 mmol?L-1 NaCl处理6 h ，己唑醇铜配合物处理小麦幼苗的脯氨酸合成增幅最大，P5CS、P5CR表达量明显增加，在一定程度上缓解了小麦幼苗的渗透胁迫伤害。
　　이기서순위배체여작산동배합획득원소조성이화적기서순동배합물。배합물대소맥충자침충처리，대맹발생장지유묘계단장기진행NaCl협박처리，통과대유묘포안산함량이급포안산합성매기인 P5CS、P5CR표체변화적측정，평개기서순동배합물대식물항염능력적영향。결과표명：소맥유묘포안산함량수착염농도적증대급협박시간적연장이증가，200 mmol?L-1 NaCl협박하，배합물처리후포안산함량최고，체55．44μg?g-1，상비기서순화작산동처리분별증가13．68％화58．45％；이100 mmol?L-1 NaCl처리6 h ，기서순동배합물처리소맥유묘적포안산합성증폭최대，P5CS、P5CR표체량명현증가，재일정정도상완해료소맥유묘적삼투협박상해。
The hexaconazole-Cu complex ,synthesized by hexaconazole and copper acetate ,was used to soak the seed of wheat to ameliorate the wheat seedlings salinity stress induced by sodium chloride (NaCl) .The effect of hexa-conazole-Cu complex on the anti-salinity stress of wheat seedlings was evaluated by proline accumulation level and the gene expression level of Δ1-pyrroline-5-carboxylate synthetase (P5CS) and Δ1-pyrroline-5-carboxylate reduc-tase (PC5R) of wheat seedlings .The results showed that in the presence of 200 mmol?L-1 NaCl ,the proline content of the 0 .03 mmol?L-1 complex treated wheat seedlings was 55 .44 μg?g-1 ,which was 13 .68% higher than that in hexa-conazole treatment ,and 58 .45% higher than that in copper acetate treatment .The gene expression levels of P5CS and P5CR were quantitated by semiquantitative RT-PCR analysis .The results showed that the hexaconazole -Cu complex enhanced the transcript levels of P5CR and P5CS .After 6 h treatment of the complex in the presence of 100 mM NaCl , the P5CS express level reached the maximum ,and was significantly higher than in hexaconazole treatment .It was obvi-ous that the hexaconazole -Cu complex enhanced the proline contents ,thereby played a protective role in the salinity stress of wheat seedlings .