CAJ | 학술논문

　　以大岩桐幼叶为材料，利用RACE技术克隆到SPY基因的全长cDNA，命名为SsSPY (Genbank：ACF96937)。序列分析表明，SsSPY包含一个完整的ORF，长度为2805 bp，编码934个氨基酸；含有SPY基因所共有的TPR保守结构域。通过蛋白序列比对，发现SsSPY与矮牵牛的PhSPY有82%的同源性，与拟南芥的SPY有81%的同源性，与大麦的HvSPY有74%的同源性。将SsSPY置于CaMV 35S启动子控制下在烟草中异位表达，转基因烟草表现出株高、花芽数、分枝数显著增加，节间变长等新表型，表明SsSPY参与了转基因烟草株形的发育及花芽的分化。
　　이대암동유협위재료，이용RACE기술극륭도SPY기인적전장cDNA，명명위SsSPY (Genbank：ACF96937)。서렬분석표명，SsSPY포함일개완정적ORF，장도위2805 bp，편마934개안기산；함유SPY기인소공유적TPR보수결구역。통과단백서렬비대，발현SsSPY여왜견우적PhSPY유82%적동원성，여의남개적SPY유81%적동원성，여대맥적HvSPY유74%적동원성。장SsSPY치우CaMV 35S계동자공제하재연초중이위표체，전기인연초표현출주고、화아수、분지수현저증가，절간변장등신표형，표명SsSPY삼여료전기인연초주형적발육급화아적분화。
Full-length cDNA of SsSPY (GenBank: ACF96937) was cloned from the young leave of Sinningia speciosa Hiern by rapid amplification of cDNA ends (RACE) technology. The cDNA sequence includes an open reading frame (ORF) of 2 805 bp length encoding 934 amino acids, the N-terminus of SsSPY contains the conserved tetratricopeptide repeats (TPR) as well. Protein secquence alignment indicated that SsSPY should be similar to PhSPY (Petunia hybrida), AtSPY (Arabidopsis thaliana) and HvSPY (Hordeum vulgare cv Himalaya) with homology of 82%, 81% and 74%, respectively. Transgenic tobacco plants ectopically expressing SsSPY exhibited novel phenotypes with plant height, branch and floral bud number increased significantly, and internodes elongated. These results indicated that SsSPY should be involved in the development of plant shape and differentiation of floral buds in transgenic tobacco.