CAJ | 학술논문

　　利用ISSR及AFLP分子标记技术研究了‘寒富’×‘四倍体嘎拉’苹果的7份三倍性后代及其亲本的遗传变异。结果表明ISSR扩增得到的遗传多样性指数中扩增多态性比率(PPB)为60.00%，观察等位基因数(Na)为1.600，有效等位基因数(Ne)为1.420，Nei's基因多样性指数(H)为0.236，Shannon信息指数(I)为0.346；均小于AFLP扩增的遗传多样性指数66.54%、1.665、1.421、0.240和0.357。根据ISSR和AFLP得到的UPGMA聚类图显示这7份三倍性杂交后代倾向于母本‘寒富’。在三倍性苹果遗传多样性检测上AFLP优于ISSR，且这7份三倍性新种质在遗传上倾向于母本遗传。为三倍性苹果育种的亲本选择提供依据。
　　이용ISSR급AFLP분자표기기술연구료‘한부’×‘사배체알랍’평과적7빈삼배성후대급기친본적유전변이。결과표명ISSR확증득도적유전다양성지수중확증다태성비솔(PPB)위60.00%，관찰등위기인수(Na)위1.600，유효등위기인수(Ne)위1.420，Nei's기인다양성지수(H)위0.236，Shannon신식지수(I)위0.346；균소우AFLP확증적유전다양성지수66.54%、1.665、1.421、0.240화0.357。근거ISSR화AFLP득도적UPGMA취류도현시저7빈삼배성잡교후대경향우모본‘한부’。재삼배성평과유전다양성검측상AFLP우우ISSR，차저7빈삼배성신충질재유전상경향우모본유전。위삼배성평과육충적친본선택제공의거。
Genetic variation of 7 triploid hybrid progenies of‘Hanfu’×‘4xGala’ were investigated based on ISSR and AFLP molecular markers in this paper. The results showed that the percentage of polymorphic bands (PPB) of ISSR was 60.00%, observed number of alleles (Na) was 1.600, effective number of alleles (Ne) was 1.420, Nei's gene diversity (H) was 0.236 and Shannon's Information index (I) was 0.346. And these were lower than that of AFLP, which were 66.54%, 1.665, 1.421, 0.240 and 0.357. The UPMGA cluster showed that most of 7 triploid hybrid progenies were tend to the female parent‘Hanfu’ based on ISSR and AFLP. Compared with ISSR, AFLP is more useful to analyze the genetic diversity of apple triploid hybrid progenies, and these triploid hybrid progenies were maternal inheritance. And these results can provide a basis for parent selecting in triploid apple breeding.