CAJ | 학술논문

　　以新疆棉区主栽品种新陆早33号和新彩棉7号为实验材料，比较不同激素组合对两者体细胞胚胎发生过程的影响，以建立高效再生体系。结果表明，新陆早33号和新彩棉7号在2,4-D+KT的激素组合下出愈率均为100%，降低2,4-D浓度有利于胚性愈伤组织的分化，分化率分别达到60.0%和7.5%；在IBA+KT的激素组合下出愈情况相对较差，分别为72.5%和85.0%；继代几次后，两个品种的胚性愈伤组织分化率可分别达到45%和55%，新陆早33号和新彩棉7号分别在DK和IK激素组合下更有利于体细胞胚胎发生过程。进一步观察发现，新彩棉7号分化形成胚状体的能力比新陆早33号更强，两品种均能在6个月内获得再生植株。再生体系的建立，为新疆棉花开展分子育种工作奠定了基础。
　　이신강면구주재품충신륙조33호화신채면7호위실험재료，비교불동격소조합대량자체세포배태발생과정적영향，이건립고효재생체계。결과표명，신륙조33호화신채면7호재2,4-D+KT적격소조합하출유솔균위100%，강저2,4-D농도유리우배성유상조직적분화，분화솔분별체도60.0%화7.5%；재IBA+KT적격소조합하출유정황상대교차，분별위72.5%화85.0%；계대궤차후，량개품충적배성유상조직분화솔가분별체도45%화55%，신륙조33호화신채면7호분별재DK화IK격소조합하경유리우체세포배태발생과정。진일보관찰발현，신채면7호분화형성배상체적능력비신륙조33호경강，량품충균능재6개월내획득재생식주。재생체계적건립，위신강면화개전분자육충공작전정료기출。
Two major cultivars, Xinluzao33 and Xincaimian7 were studied for establishing efficient regeneration system, and the procedures of somatic embryogenesis were compared in different hormone combination. Calli induction rates of the two varieties reached 100% in 2,4-D+KT hormone combination; the induction rates of embryogenic calli of Xinluzao33 and Xincaimian7 were significantly enhanced through reducing the concentration of 2,4-D, and could reach 60.0%and 7.5%, respectively. Calli induction rates of the two varieties were lower in IBA+KT hormone combination, reached 72.5%and 85%in Xinluzao33 and Xincaimian7, respectively. Subculture for several months late, the induction rates of embryogenic calli of Xinluzao33 and Xincaimian7 could reach 45%and 55%, respectively. DK and IK hormone combination were more suitable for Xinluzao33 and Xincaimian7, respectively. Further investi-gation suggested Xincaimian7 showed strong embryogenic capacity than Xinluzao33. Regenerated plants were obtained from the two cultivars within 6 months.