CAJ | 학술논문

　　为有效建立化杀杂交油菜品种的纯度鉴定方法，本研究利用简单重复序列(SSR)技术，对秦杂油19及其亲本进行了分子鉴定，从800对SSR引物中筛选出了5对带型清晰、扩增效果好且能将亲本与杂交种完全区别的引物。利用其中的SR767和SR790两引物对三者的单株进行了分析验证，三者的扩增图谱与标准图谱对应率极高，分别为97.33%、98.00%、100%和98.67%、96.67%、98.00%；利用两对SSR引物对秦杂油19的栽培品种进行分别鉴定和比较研究，结果显示两对不同引物的SSR分子鉴定结果的单株吻合率96.67%~98.32%之间，结果表明，利用SSR分子标记可以用于杂交油菜纯度鉴定。
　　위유효건립화살잡교유채품충적순도감정방법，본연구이용간단중복서렬(SSR)기술，대진잡유19급기친본진행료분자감정，종800대SSR인물중사선출료5대대형청석、확증효과호차능장친본여잡교충완전구별적인물。이용기중적SR767화SR790량인물대삼자적단주진행료분석험증，삼자적확증도보여표준도보대응솔겁고，분별위97.33%、98.00%、100%화98.67%、96.67%、98.00%；이용량대SSR인물대진잡유19적재배품충진행분별감정화비교연구，결과현시량대불동인물적SSR분자감정결과적단주문합솔96.67%~98.32%지간，결과표명，이용SSR분자표기가이용우잡교유채순도감정。
To establish an effective method for purity identification of chemical emasculation hybrid B. napus, 800 pairs of SSR primers were screened in this study, and 5 of them were able to produce clear pattems and distinguish the hybrid Qinzayou19 from its parents effectively. The single-plant of three lines were verified by using primers SR767 and primer SR790. A high consistency was found between application pattern of the three and the standard map, which are 97.33%, 98.00%, 100% and 98.67%, 96.67%, 98.00% respectively; then two pairs of primers were used to identify the Qinzayou19 grow in the field, and the consistency compare with single-plant of two pairs of primers were between 96.67%and 98.32%. The result showed that SSR technology can be used for hybrid seed purity identification.