CAJ | 학술논문

　　为探究干旱胁迫对转玉米高光效基因ZmPEPC与ZmPPDK拟南芥的影响，本研究选用2个转PEPC基因株系(PC90, PC73)，2个转PPDK基因株系(PK17, PK26)，1个转PEPC+PPDK基因株系(PCK110)和对照株系(GLC)为试验材料。在幼苗期分别用150 mmol/L和250 mmol/L甘露醇(Mannitol)模拟干旱胁迫处理，测定幼苗根长，结果显示，在胁迫处理下，转PEPC、PPDK和PEPC+PPDK基因拟南芥幼苗的平均相对根长分别较对照增加了43.8%，48.4%和50.6%。在成株期进行干旱胁迫处理，测定莲座叶片的叶绿素荧光参数，叶绿素、可溶性糖、可溶性蛋白、丙二醛含量和成株死亡率，结果显示，在胁迫处理下，供试材料F0增加， Fv/Fm减小，对照F0增幅最大，为20.4%，Fv/Fm最小，为0.78；转PEPC、PPDK和PEPC+PPDK基因拟南芥株系平均相对叶绿素含量分别较对照增加12.6%，15.4%和23.1%；可溶性糖含量分别较对照增加3.0%，16.3%和24.4%；可溶性蛋白含量分别较对照增加0.6%，10.8%和15.4%；丙二醛含量较对照降低，分别为对照的93.5%，90.3%和84.10%；死亡率分别为12.3%，13.3%和6.7%，极显著低于对照(87.7%)(P<0.01)。上述结果表明，玉米高光效基因ZmPEPC和ZmPPDK可增强拟南芥抵抗干旱胁迫的能力，且这两个基因的共表达可能存在协同作用。
　　위탐구간한협박대전옥미고광효기인ZmPEPC여ZmPPDK의남개적영향，본연구선용2개전PEPC기인주계(PC90, PC73)，2개전PPDK기인주계(PK17, PK26)，1개전PEPC+PPDK기인주계(PCK110)화대조주계(GLC)위시험재료。재유묘기분별용150 mmol/L화250 mmol/L감로순(Mannitol)모의간한협박처리，측정유묘근장，결과현시，재협박처리하，전PEPC、PPDK화PEPC+PPDK기인의남개유묘적평균상대근장분별교대조증가료43.8%，48.4%화50.6%。재성주기진행간한협박처리，측정련좌협편적협록소형광삼수，협록소、가용성당、가용성단백、병이철함량화성주사망솔，결과현시，재협박처리하，공시재료F0증가， Fv/Fm감소，대조F0증폭최대，위20.4%，Fv/Fm최소，위0.78；전PEPC、PPDK화PEPC+PPDK기인의남개주계평균상대협록소함량분별교대조증가12.6%，15.4%화23.1%；가용성당함량분별교대조증가3.0%，16.3%화24.4%；가용성단백함량분별교대조증가0.6%，10.8%화15.4%；병이철함량교대조강저，분별위대조적93.5%，90.3%화84.10%；사망솔분별위12.3%，13.3%화6.7%，겁현저저우대조(87.7%)(P<0.01)。상술결과표명，옥미고광효기인ZmPEPC화ZmPPDK가증강의남개저항간한협박적능력，차저량개기인적공표체가능존재협동작용。
Transformation and high efficiency expression of key enzymes gene of C4 photosynthetic character-istics into C3 plants are supposed to enhance the photosynthetic efficiency and seed yield for C3 crop. Phosphoen-olpyruvate carboxylase (PEPC) and Pyruvate orthophosphate dikinase (PPDK) are known to play the key roles in C4 pathway, and it has been confirmed that the photosynthesis and enzymes activities of the PEPC or PPDK were elevated by introducing PEPC or PPDK gene into C3 plants. In order to determine the drought-Stress tolerance of the trangenic Arabidopsis containing ZmPEPC and ZmPPDK, five T6 generation purified lines of transgenic plants (2 lines contain the PEPC, 2 lines contain the PPDK and 1 line contains PEPC+PPDK) and wild Arabidopsis were sele-cted to study their drought-Stress tolerance. When grown in the 1/2MS medium contained 150 mmol/L, 250 mmol/L mannitol, as simulation of drought-stress conditions, The average relative root lengths in transgenic lines PEPC, PPDK, PEPC+PPDK were 43.8%, 48.4%and 50.6%longer than those in wild type plants, respectively. When 15 days after drought-stress treatment, F0 was increased and Fv/Fm was decreased, the increased range of wild type plants was biggest which was 20.4%, Fv/Fm of wild type plants was minimum which was 0.78. The average contents of chlorophyll in transgenic lines PEPC, PPDK, PEPC+PPDK were 112.6%, 115.4%, 123.1% times of that in wild type plants, respectively;the average values of soluble sugar and soluble protein contents were 3.0%, 16.3%, 24.4% and 0.6%, 10.8%, 15.4% higher than wild plants, respectively; the malondialdehyde content of transgenic lines was lower than that of wild plants, the average values were 93.5%, 90.3%and 84.1%of that in wild type plants, respectively. When 25 days after drought-stress treatment and re-water, 5 days later, the death rates of transgenic lines PEPC, PPDK, and PEPC+PPDK were 12.3%, 13.3%, and 6.7%, respectively, which were extremely significantly lower than that of wild type plants (87.7%). Therefore, it is supposed that PEPC and PPDK might enhance the ability of resist to drought-stress in Arabidopsis thaliana, and co-expression of PEPC and PPDK had synergistic effect on stress tolerance.