世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2014年
1期
71-74
,共4页
吕祥%冯彦军%夏英%刘静%侯风刚%李雁%任建琳%陈伟杰
呂祥%馮彥軍%夏英%劉靜%侯風剛%李雁%任建琳%陳偉傑
려상%풍언군%하영%류정%후풍강%리안%임건림%진위걸
淫羊藿素%人脐静脉内皮细胞%血管新生%血管生成抑制剂
淫羊藿素%人臍靜脈內皮細胞%血管新生%血管生成抑製劑
음양곽소%인제정맥내피세포%혈관신생%혈관생성억제제
Icaritin,Human umbilical vein endothelial cell%Angiogenesis%Angiogenesis inhibitor
目的:探讨淫羊藿素对人脐静脉内皮细胞( HUVEC)血管生成的抑制作用及其机制。方法:体外培养人脐静脉内皮细胞,分别观察淫羊藿素对其增殖、迁移及其小管形成的影响。采用酶联免疫吸附法检测血管内皮细胞生长因子( VEGF )和色素上皮衍生因子( PEDF)的含量。结果:经淫羊藿素作用48 h后,未见对内皮细胞增殖有影响。淫羊藿素高浓度组( ICT110-6 mol/L)、中浓度组(ICT210-7 mol/L)、低浓度组(ICT310-8 mol/L)和沙利度胺(TLD)组HUVEC细胞迁移数目(4.67±1.26)、(10.48±3.15)、(21.06±6.83)和(19.15±6.03)个,明显低于空白对照(Control)组(41.38±7.78)个(P<0.01)。 ICT1、ICT2、ICT3及TLD组小管形成的面积分别为(5867.45±925.36)、(1627.33±288.56)、(735.73±325.65)和(2933.24±741.43)μm2/视野,均低于Control组(7883.69±1034.85)μm2/视野(P<0.01)。经淫羊藿素处理后,HUVEC细胞分泌VEGF功能明显下降,而分泌PEDF功能明显升高。结论:体外实验结果表明淫羊藿素具有抑制HUVEC细胞血管生成的作用,这种效应与血管内皮细胞VEGF的活性降低及其合成受到抑制,同时对PEDF活性升高及其合成受到促进作用有关。
目的:探討淫羊藿素對人臍靜脈內皮細胞( HUVEC)血管生成的抑製作用及其機製。方法:體外培養人臍靜脈內皮細胞,分彆觀察淫羊藿素對其增殖、遷移及其小管形成的影響。採用酶聯免疫吸附法檢測血管內皮細胞生長因子( VEGF )和色素上皮衍生因子( PEDF)的含量。結果:經淫羊藿素作用48 h後,未見對內皮細胞增殖有影響。淫羊藿素高濃度組( ICT110-6 mol/L)、中濃度組(ICT210-7 mol/L)、低濃度組(ICT310-8 mol/L)和沙利度胺(TLD)組HUVEC細胞遷移數目(4.67±1.26)、(10.48±3.15)、(21.06±6.83)和(19.15±6.03)箇,明顯低于空白對照(Control)組(41.38±7.78)箇(P<0.01)。 ICT1、ICT2、ICT3及TLD組小管形成的麵積分彆為(5867.45±925.36)、(1627.33±288.56)、(735.73±325.65)和(2933.24±741.43)μm2/視野,均低于Control組(7883.69±1034.85)μm2/視野(P<0.01)。經淫羊藿素處理後,HUVEC細胞分泌VEGF功能明顯下降,而分泌PEDF功能明顯升高。結論:體外實驗結果錶明淫羊藿素具有抑製HUVEC細胞血管生成的作用,這種效應與血管內皮細胞VEGF的活性降低及其閤成受到抑製,同時對PEDF活性升高及其閤成受到促進作用有關。
목적:탐토음양곽소대인제정맥내피세포( HUVEC)혈관생성적억제작용급기궤제。방법:체외배양인제정맥내피세포,분별관찰음양곽소대기증식、천이급기소관형성적영향。채용매련면역흡부법검측혈관내피세포생장인자( VEGF )화색소상피연생인자( PEDF)적함량。결과:경음양곽소작용48 h후,미견대내피세포증식유영향。음양곽소고농도조( ICT110-6 mol/L)、중농도조(ICT210-7 mol/L)、저농도조(ICT310-8 mol/L)화사리도알(TLD)조HUVEC세포천이수목(4.67±1.26)、(10.48±3.15)、(21.06±6.83)화(19.15±6.03)개,명현저우공백대조(Control)조(41.38±7.78)개(P<0.01)。 ICT1、ICT2、ICT3급TLD조소관형성적면적분별위(5867.45±925.36)、(1627.33±288.56)、(735.73±325.65)화(2933.24±741.43)μm2/시야,균저우Control조(7883.69±1034.85)μm2/시야(P<0.01)。경음양곽소처리후,HUVEC세포분비VEGF공능명현하강,이분비PEDF공능명현승고。결론:체외실험결과표명음양곽소구유억제HUVEC세포혈관생성적작용,저충효응여혈관내피세포VEGF적활성강저급기합성수도억제,동시대PEDF활성승고급기합성수도촉진작용유관。
Objective:To investigate the suppressive effect of Icaritin on angiogenesis of human umbilical vein endothelial cells ( HU-VEC) and its associated mechanisms.Methods:HUVEC cells were cultured in vitro, and we observed the effects of Icaritin on its prolif-eration, migration and tube formation .The content of vascular endothelial growth factor ( VEGF) and pigment epithelium-derived factor (PEDF) was detected by ELISA.Results:Icaritin had no significant effect on the proliferation of HUVEC cells after 48h treatment.The number of migrated cells in Icaritin high concentration group ( ICT1 10 -6 mol/l) , middle concentration group ( ICT2 10 -7 mol/l) , low concentration group (ICT3 10 -8 mol/l) and Thalidomide (TLD) group were 4.67 ±1.26, 10.48 ±3.15, 21.06 ±6.83, 19.15 ±6.03 respectively, and all of which were lower compared with the control group (41.38 ±7.78) (P<0.01).The area of tube formation in ICT1、ICT2、ICT3 and TLD were separately 5 867.45 ±925.36, 1 627.33 ±288.56, 735.73 ±325.65, 2 933.24 ±741.43μm2 per vi-sion, all of which were lower than the control group (7 883.69 ±1 034.85 μm2 per vision) (P<0.01).After Icaritin treatment, HU-VEC cells had a significant lower ability to secret VEGF , but asfor PEDF , it was on the opposite situation .Conclusion:Icaritin can in-hibit the angiogenesis of HUVEC cells in vitro .Such effect may be related to the decreased activity and synthesis of VEGF in vascular en -dothelial cells , and also associated with the increased activity and synthesis of PEDF .