CAJ | 학술논문

　　本研究旨在筛选新型免疫原性好、广谱的优秀 H9亚型禽流感病毒疫苗株。通过 H9N2分子流行病学调查对分离得到的37株禽流感 H9N2亚型病毒进行 HA 基因分子遗传进化及其关键位点分析，利用交叉 HI 效价及鸡胚中和试验分析毒株间毒力及其相关性，制备疫苗用毒株进行免疫攻毒保护试验。结果显示，从37株病毒中选出12株病毒作为代表，分为3个分支，同分支之间交叉 HI 效价及中和效价最高。HI 试验及鸡胚中和试验均显示同分支毒株间的相关系数高。免疫攻毒保护试验显示同分支疫苗株安全性高。表明我国禽流感 H9N2亚型情况复杂，且疫苗保护效果与疫苗株的抗原匹配性密切相关，生产多种分支 H9疫苗株联合更有利于控制 H9型禽流感，
　　본연구지재사선신형면역원성호、엄보적우수 H9아형금류감병독역묘주。통과 H9N2분자류행병학조사대분리득도적37주금류감 H9N2아형병독진행 HA 기인분자유전진화급기관건위점분석，이용교차 HI 효개급계배중화시험분석독주간독력급기상관성，제비역묘용독주진행면역공독보호시험。결과현시，종37주병독중선출12주병독작위대표，분위3개분지，동분지지간교차 HI 효개급중화효개최고。HI 시험급계배중화시험균현시동분지독주간적상관계수고。면역공독보호시험현시동분지역묘주안전성고。표명아국금류감 H9N2아형정황복잡，차역묘보호효과여역묘주적항원필배성밀절상관，생산다충분지 H9역묘주연합경유리우공제 H9형금류감，
Objective The purpose of this study is to select a new H9N2 AIV vaccine strain with good immunogenicity. Methods 37 H9N2 subtype avian influenza virus isolates were analyzed for HA gene molecular genetic evolution and its key sites. Cross-HI titers and chicken embryo neutralization test were used to check virulence and the relevance between the different isolates,and the strains for vaccine production were tested for their immune protection.Results 12 representative strains in the 37 viruses were divided into three subbranches,cross-HI titers and neutralization titers of the same subbranch were the highest. From relevance of HI test and the chicken embryo neutralization test,the correlation coefficient in same subbranch strains were higher than in different subbranch. In the immune protection test,the strains showed safety in the same. Conclusion H9 vaccine with differnet subtype strains is more effective to preventing H9 avain flu. Preventive effect of vaccine is tightly related with antigen matched to the vaccine strain.