CAJ | 학술논문

　　为了获得水貂的有益菌种，及研制毛皮动物益生菌制剂提供基础，并为进一步深入研究枯草芽孢杆菌提供参考，从健康水貂的肠道中分离纯化出1株枯草芽孢杆菌，编号为 P2，通过形态学观察、生理生化试验以及16S rRNA 序列分析及同源性比较进行综合鉴定，结果显示菌株 P2与菌株 Bacillus subtilis CICC 10023（GU980947.1）亲缘关系最近，同源性达到了99.9%，因此鉴定 P2菌株为枯草芽孢杆菌。
　　위료획득수초적유익균충，급연제모피동물익생균제제제공기출，병위진일보심입연구고초아포간균제공삼고，종건강수초적장도중분리순화출1주고초아포간균，편호위 P2，통과형태학관찰、생리생화시험이급16S rRNA 서렬분석급동원성비교진행종합감정，결과현시균주 P2여균주 Bacillus subtilis CICC 10023（GU980947.1）친연관계최근，동원성체도료99.9%，인차감정 P2균주위고초아포간균。
In order to obtain mink beneficial bacteria and to provide theoretical basis for developing probiotic preparations,one bacillus strain was isolated and purified from the intestines of healthy mink named P2. Morphological observation,physiological and biochemical tests and 16S rRNA gene sequence analysis,and homology comparison were conducted. The results showed that Strain P2 and strains of Bacillus subtilis CICC 10023(GU980947.1) had the nearest genetic relationship with the homology was upto 99.9% and the P2 was identified as Bacillus subtilis strain.