CAJ | 학술논문

背景：哺乳动物细胞外基质组成的生物型修复材料，在盆底组织修复及功能重建方面表现出较大潜力，但细菌感染对生物型修复材料的功能破坏是其中的一个较大问题。 　　目的：制备具备抗菌功能的生物型盆底修复材料。 　　方法：以壳聚糖为基质材料，通过静电吸附和自凝聚纳米粒制备技术获得包载有不同质量浓度替加环素(25，50，100 g/L)的壳聚糖大分子纳米粒混合液，再通过表面涂层技术将其涂覆于细胞外基质材料表面，制得抗菌细胞外基质材料。 　　结果与结论：红外光谱显示，抗菌细胞外基质材料出现壳聚糖的典型糖环骨架振动峰，并且在3359 cm-1左右峰明显增宽，表明抗菌剂复合涂层修饰成功，由于替加环素的用量非常小，其红外图谱中未能显示其特征吸收峰。扫描电镜观察显示，抗菌细胞外基质材料保存了细胞外基质材料多层排列有序纤维丝构成的主体结构，并且在微丝表面和微丝之间还明显附着一层蓬松鳞片状涂层物质，材料微表面三维孔洞变小，但仍维持多孔连通特性。抑菌圈实验显示，抗菌细胞外基质材料对大肠杆菌和金黄色葡萄球菌具有良好的抗菌性能，并且随着抗菌剂浓度的增大，抑菌圈直径显著增加。
배경：포유동물세포외기질조성적생물형수복재료，재분저조직수복급공능중건방면표현출교대잠력，단세균감염대생물형수복재료적공능파배시기중적일개교대문제。 　　목적：제비구비항균공능적생물형분저수복재료。 　　방법：이각취당위기질재료，통과정전흡부화자응취납미립제비기술획득포재유불동질량농도체가배소(25，50，100 g/L)적각취당대분자납미립혼합액，재통과표면도층기술장기도복우세포외기질재료표면，제득항균세포외기질재료。 　　결과여결론：홍외광보현시，항균세포외기질재료출현각취당적전형당배골가진동봉，병차재3359 cm-1좌우봉명현증관，표명항균제복합도층수식성공，유우체가배소적용량비상소，기홍외도보중미능현시기특정흡수봉。소묘전경관찰현시，항균세포외기질재료보존료세포외기질재료다층배렬유서섬유사구성적주체결구，병차재미사표면화미사지간환명현부착일층봉송린편상도층물질，재료미표면삼유공동변소，단잉유지다공련통특성。억균권실험현시，항균세포외기질재료대대장간균화금황색포도구균구유량호적항균성능，병차수착항균제농도적증대，억균권직경현저증가。
BACKGROUND:The biological extracellular matrix materials become the focus of pelvic floor repair materials research because of its excellent biocompatibility and mechanical compatibility. However, bacterial infection can damage the function of biological repair materials. OBJECTIVE:To prepare the biologic antimicrobial materials for pelvic tissue function repair. METHODS:Chitosan and tigecycline-loaded chitosan nanoparticles suspension was prepared by the electrostatic adsorption and self-aggregation of nanoparticles preparation techniques. Then the suspension was coated onto the surface of extracellular matrix materials. The morphological examination was performed by scanning electron microscope. The antibacterial property was detected by solid plate microbial culture method. RESULTS AND CONCLUSION:Infrared spectra displayed that aromatic ring skeleton vibration peak of chitosan occurred on the biologic antimicrobial materials, and it was significantly widened at about 3 359 cm-1, indicating the composite coating was successful to modify the surface of extracellular matrix materials. As a very smal dose of tigecycline, there was no characteristic absorption peak on the infrared spectra. Scanning electron microscopy showed the porous structure of the material surface with some nanoparticles adhesion. The prepared materials had good antibacterial properties on Staphylococcus aureus and Escherichia coli, and the inhibition zone diameter was significantly increased with the increasing concentration of antibacterial agent. The biologic antimicrobial materials for pelvic tissue function repair were prepared successful y.