CAJ | 학술논문

利用豇豆SSR引物筛选在豌豆具通用性的SSR引物。13对豇豆SSR引物中检测出8对引物在豌豆中可扩增，其中5对SSR引物表现出多态性，进而分析11个豌豆品种和5个豇豆品种的遗传多样性与遗传关系。结果表明：平均等位基因数、平均有效等位基因数、平均Shannon指数，在豌豆品种群中分别为2.00、1.64、0.55，豇豆品种群中分别为2.00、1.80、0.53，上述遗传参数反映出供试豌豆品种和豇豆品种具有中等水平的遗传多样性；利用UPGMA聚类分析，能将豌豆品种群和豇豆品种群分为两类，且每个品种群内的各个体之间能进行区分，尤其是两个豌豆品种亚群的聚类结果与其地理来源基本一致。
이용강두SSR인물사선재완두구통용성적SSR인물。13대강두SSR인물중검측출8대인물재완두중가확증，기중5대SSR인물표현출다태성，진이분석11개완두품충화5개강두품충적유전다양성여유전관계。결과표명：평균등위기인수、평균유효등위기인수、평균Shannon지수，재완두품충군중분별위2.00、1.64、0.55，강두품충군중분별위2.00、1.80、0.53，상술유전삼수반영출공시완두품충화강두품충구유중등수평적유전다양성；이용UPGMA취류분석，능장완두품충군화강두품충군분위량류，차매개품충군내적각개체지간능진행구분，우기시량개완두품충아군적취류결과여기지리래원기본일치。
Aimed to screen out transferable SSR(simple sequence repeat)molecular markers from cowpea (Vigna unguiculata) to pea (Pisum sativum). Using thirteen cowpea SSR primer pairs, eight primer pairs were successfully cross-amplified in pea. Five of the eight primer pairs exhibited polymorphism,and then to study the genetic variation of eleven pea cultivars and five cowpea culti?vars. Results showed that genetic parameters of the average number of alleles,the average effective number of alleles and the average Shannon′s index are 2. 00,1. 64,0. 55 in the pea cultivar group, and 2. 00,1. 80,0. 53 in the cowpea cultivar group,respectively. These indicated that a moderate level of genetic diversity was revealed in the two cultivar groups. In addtion,UPGMA clustering showed that the pea cultivar group and the cowpea cultivar group clustered into their own branches, respectively. Within each group,most of the samples can be distinguished from each other. Especial?ly,within the pea cultivar group,all the samples can be divided into two subgroups,which are con?sistent with their geographical origin.