CAJ | 학술논문

　　目的：研究大孔吸附树脂富集纯化川贝母总生物碱的工艺参数及条件。方法：7种不同类型的大孔吸附树脂被研究，以总生物碱的吸附率及解吸率为考察指标，探索吸附和解吸的条件，筛选出纯化总生物碱的最佳工艺。结果：HPD-100大孔树脂对川贝母总生物碱有较好的富集纯化性能。最佳工艺条件为：上样药液质量浓度为0.293g生药/mL，pH值为11.6，6BV 的80%乙醇洗脱总生物碱，洗脱体积流量为2BV/h。按照此工艺条件纯化的总生物碱纯度达到51.92%，收率76.65%。结论：建立HPD-100大孔吸附树脂富集纯化川贝母总生物碱的方法简单，可靠，重现性好，适合富集纯化川贝母总生物碱，为其它种贝母总生物碱的富集纯化提供参考。
　　목적：연구대공흡부수지부집순화천패모총생물감적공예삼수급조건。방법：7충불동류형적대공흡부수지피연구，이총생물감적흡부솔급해흡솔위고찰지표，탐색흡부화해흡적조건，사선출순화총생물감적최가공예。결과：HPD-100대공수지대천패모총생물감유교호적부집순화성능。최가공예조건위：상양약액질량농도위0.293g생약/mL，pH치위11.6，6BV 적80%을순세탈총생물감，세탈체적류량위2BV/h。안조차공예조건순화적총생물감순도체도51.92%，수솔76.65%。결론：건립HPD-100대공흡부수지부집순화천패모총생물감적방법간단，가고，중현성호，괄합부집순화천패모총생물감，위기타충패모총생물감적부집순화제공삼고。
Objective To study technological parameters of enriching and purifying total alkaloids from Fritillariae Cirrhosae Bulbus by macroporous resins. Methods The adsorptive and desorption ratio of the total al-kaloids was determined by UV spectra. The optimized macroporous resins were selected by studying 7 kinds of macroporous resins. The optimized technological parameter was screened out by further studying absorption and desorption. Results HPD-100 macroporous resins was selected as the best material in enrichment and purifica-tion of total alkaloids for Fritillariae Cirrhosae Bulbus. The optimized technological paramaters was 0.293g/mL concentration, pH value 11.6, delution by 6BV, 80%alcohol in 2BV/h. The content of total alkaloids obtained was 51.92%, and the yield was 76.65%. Conclusion Using HPD-100 macroporous resins to enrich total alkaloids in Fritillariae Cirrhosa Bulbus is easy, relable and good reproductively, which could also provide a reference for oth-er Fritillariae species.