CAJ | 학술논문

　　为研究接骨草成分之一绿原酸(CGA)对成骨细胞MC3T3-E1活性的影响,采用MTT法检测11.02,22.05,44.09,88.19μmol/L CGA对成骨细胞增殖率,碱性磷酸酶(ALP)试剂盒检测成骨细胞ALP 活性,实时定量PCR检测成骨细胞骨相关基因的表达.结果表明：11.02~88.19μmol/L CGA对成骨细胞增殖具有明显的促进作用.培养2 d,44.09μmol/L CGA能促进ALP表达上调；22.05,44.09μmol/L CGA能促进ALP、Runx2、Osterix、c-jun和c-fos基因的表达；培养6 d,44.09μmol/L CGA能促进c-fos基因的表达；在整个培养过程中,I型胶原( Col-I)基因的表达具有浓度和时间依赖性.故CGA具有一定的成骨活性,是接骨草的成骨活性成分之一.
　　위연구접골초성분지일록원산(CGA)대성골세포MC3T3-E1활성적영향,채용MTT법검측11.02,22.05,44.09,88.19μmol/L CGA대성골세포증식솔,감성린산매(ALP)시제합검측성골세포ALP 활성,실시정량PCR검측성골세포골상관기인적표체.결과표명：11.02~88.19μmol/L CGA대성골세포증식구유명현적촉진작용.배양2 d,44.09μmol/L CGA능촉진ALP표체상조；22.05,44.09μmol/L CGA능촉진ALP、Runx2、Osterix、c-jun화c-fos기인적표체；배양6 d,44.09μmol/L CGA능촉진c-fos기인적표체；재정개배양과정중,I형효원( Col-I)기인적표체구유농도화시간의뢰성.고CGA구유일정적성골활성,시접골초적성골활성성분지일.
To investigate the effect of chlorogenic acid(CGA), one of the components of Sambucus chinensis, on the osteogenic activity of cultured osteoblasts, osteoblasts were treated with 11. 02,22. 05, 44. 09, 88. 19 μmol/L CGA, respectively. MTT assay, alkaline phosphate(ALP) kit, real-time PCR were used to measure the proliferation rate, ALP activity and expression of bone-related genes in osteoblasts. The results showed that 11. 02 ~88. 19 μmol/L CGA could promote the proliferation rate remarkably. The activity of ALP in osteoblasts was up-regulated when treated with 44. 09μmol/L CGA for 2 d. The expression of bone-related genes such as ALP、Runx2、Osterix、c-jun and c-fos could be enhanced when treated with 22. 05,44. 09 μmol/L CGA for 2 d. The expression of c-fos could be promoted when treated with 44. 09 μmol/L CGA for 6 days. In the whole process, the expression of Col-I was increased in a time-dependent and concentration-dependent manner . Therefore, CGA showed osteogenic activity , which demonstrated that it was one of the osteogenic active ingredients of Sambucus chinensis.