CAJ | 학술논문

　　采用体外实验筛选益生菌结合16S rDNA序列分析法,从124株不具有溶血作用的皱纹盘鲍(Haliotis discus hannai)肠道细菌中筛选得到5株潜在益生菌并进行了分子鉴定,进一步对5株潜在益生菌进行了安全性实验及体内饲喂实验。结果表明,2株具有拮抗哈维弧菌和灿烂弧菌能力的潜在益生菌分别被鉴定为Shewanella sp.(WA64)和Shewanella sp.(WA65),一株产海藻酸酶和淀粉酶的潜在益生菌被鉴定为Vibrio sp.(WA51),产蛋白酶潜在益生菌被鉴定为 Bacillus sp.(FA12),产琼脂酶潜在益生菌被鉴定为Tamlana sp.(FA86)；安全性实验表明5株潜在益生菌在107cfu/ml下对皱纹盘鲍没有明显的毒害作用；通过体内饲喂实验发现,潜在益生菌 WA64、WA65的复合作用能够显著提高幼鲍增重率和存活率(P<0.05),并在生产条件下能够明显降低幼鲍的死亡量。经抗生素敏感性实验, WA64菌株对15种抗生素均敏感或中度敏感, WA65菌株仅对庆大霉素和链霉素2种抗生素产生耐药。
　　채용체외실험사선익생균결합16S rDNA서렬분석법,종124주불구유용혈작용적추문반포(Haliotis discus hannai)장도세균중사선득도5주잠재익생균병진행료분자감정,진일보대5주잠재익생균진행료안전성실험급체내사위실험。결과표명,2주구유길항합유호균화찬란호균능력적잠재익생균분별피감정위Shewanella sp.(WA64)화Shewanella sp.(WA65),일주산해조산매화정분매적잠재익생균피감정위Vibrio sp.(WA51),산단백매잠재익생균피감정위 Bacillus sp.(FA12),산경지매잠재익생균피감정위Tamlana sp.(FA86)；안전성실험표명5주잠재익생균재107cfu/ml하대추문반포몰유명현적독해작용；통과체내사위실험발현,잠재익생균 WA64、WA65적복합작용능구현저제고유포증중솔화존활솔(P<0.05),병재생산조건하능구명현강저유포적사망량。경항생소민감성실험, WA64균주대15충항생소균민감혹중도민감, WA65균주부대경대매소화련매소2충항생소산생내약。
Five strains of potential probiotics isolated from 124 non-hemolysis strains of the GI of abalone in vitro were identified by 16S rDNA sequence analysis. In addition, pathogenicity and feeding experiment were conducted. Results show that Strain WA64 and WA65 which exhibit inhibitory effects against Vibrio harveyi, Vibrio splendidus are identified as Shewanella;WA51 produced amylase and alginase is identified as Vibrio;FA123 produced protease is identified as Ba-cillus; FA86 produced agarase is identified as Tamlana. Potential probiotics are safe to abalone under 107cfu/ml. Feeding experiment showed the weight gain ratio and survival rate were significantly increased in treatment supplemented with WA64 and WA65 (P<0.05). Furthermore, the effects of WA64 and WA65 in pilot scale trials show the mortality of juvenile small abalone decreases obviously. Antibiotic sensitivity assays revealed that strain WA64 was sensitive or medium sensi-tive to the 15 antibiotics tested, and WA65 was resistant to Gentamicin and Streptomycin.