CAJ | 학술논문

　　以初始体重为(13.0±0.3)g 的斜带石斑鱼(Epinephelus coioides)为实验对象,用铜添加量为0、1、3、5、10和20mg/kg的实验饲料投喂斜带石斑鱼8周,研究斜带石斑鱼的铜需求量。结果表明,饲料中添加0-20mg/kg铜对石斑鱼生长、饲料效率、存活率、形态学指标和红细胞比容没有显著性影响。随饲料铜水平的上升,斜带石斑鱼全鱼和肝脏的铜含量呈先上升后平稳的趋势,当饲料铜添加量达到或超过3mg/kg时,石斑鱼全鱼和肝脏的铜含量趋于平稳。折线模型表明,饲料中铜总含量为5-5.5mg/kg时,石斑鱼全鱼和肝脏的铜含量能够达到饱和。当饲料中铜总含量≥6.00mg/kg时,全鱼的铁、锰、锌含量显著下降。因此,根据全鱼和肝脏铜含量的折线模型分析,考虑到饲料铜对全鱼铁、锰、锌含量的影响,作者认为当基础饲料铜含量为1.50mg/kg 时,饲料中的最适铜添加量为3mg/kg,饲料中铜总量为5-5.5mg/kg左右。
　　이초시체중위(13.0±0.3)g 적사대석반어(Epinephelus coioides)위실험대상,용동첨가량위0、1、3、5、10화20mg/kg적실험사료투위사대석반어8주,연구사대석반어적동수구량。결과표명,사료중첨가0-20mg/kg동대석반어생장、사료효솔、존활솔、형태학지표화홍세포비용몰유현저성영향。수사료동수평적상승,사대석반어전어화간장적동함량정선상승후평은적추세,당사료동첨가량체도혹초과3mg/kg시,석반어전어화간장적동함량추우평은。절선모형표명,사료중동총함량위5-5.5mg/kg시,석반어전어화간장적동함량능구체도포화。당사료중동총함량≥6.00mg/kg시,전어적철、맹、자함량현저하강。인차,근거전어화간장동함량적절선모형분석,고필도사료동대전어철、맹、자함량적영향,작자인위당기출사료동함량위1.50mg/kg 시,사료중적최괄동첨가량위3mg/kg,사료중동총량위5-5.5mg/kg좌우。
This study was conducted to investigate the copper requirement of juvenile grouper, Epinephelus coioides. Six diets were prepared by supplementing a casein-gelatin-based diet with 0, 1, 3, 5, 10 and 20mg/kg copper from CuSO4·5H2O. Grouper with an initial weight of (13.0±0.3)g were fed to satiation for 8 weeks. Growth performance, feed utilization, morphological indicators and hematocrit value of juvenile grouper were not significantly affected by dietary copper supplements ranging from 0 to 20mg/kg. Copper concentration of whole body and liver responded linearly to die-tary copper supplement up to 3mg/kg and then reached a plateau. Broken-line analysis indicated that dietary copper of 5-5.5mg/kg satisfied whole body and liver copper storage. Whole body Fe, Mn and Zn were significantly lower when dietary total copper≥6mg/kg. Liver Fe, Mn and Zn were not significantly affected by dietary copper supplement ranging from 0 to 20mg/kg. Concerning broken-line analysis of whole body and liver Cu, and the interaction effect of Cu supplement on whole body Fe, Mn and Zn concentration, we estimate that copper supplement of 3mg/kg might be optimum to the basal diet containing 1.50mg/kg Cu, providing dietary total copper of 5-5.5mg/kg.