CAJ | 학술논문

　　采用常规急性实验方法，研究了不同干露胁迫条件对脊尾白虾(Exopalaemon carinicauda)成活率的影响。结果表明，保持虾体湿润并用冰块降温的 P 组干露胁迫12h 后成活率为75%，显著高于其它各实验组(P<0.05)。采用实时荧光定量PCR方法研究了不同干露胁迫条件对脊尾白虾血细胞和肝胰腺组织中热休克蛋白70(HSP70)和ferritin基因表达的影响。干露胁迫能诱导脊尾白虾血细胞、肝胰腺 HSP70基因的表达上调，肝胰腺中的高表达时间相对血细胞中出现的较早；干露胁迫能诱导湿润低温P组脊尾白虾血细胞和肝胰腺ferritin基因的表达上调，并显著高于对照组(P<0.05)，而且各组织 ferritin 基因表达的上调时间具有差异性，血细胞最先上调。其余实验组脊尾白虾各组织ferritin基因表达均下调，并显著低于对照组(P<0.05)。上述结果表明，在脊尾白虾受干露胁迫的耐受范围内， HSP70和ferritin基因发挥抗氧化功能。
　　채용상규급성실험방법，연구료불동간로협박조건대척미백하(Exopalaemon carinicauda)성활솔적영향。결과표명，보지하체습윤병용빙괴강온적 P 조간로협박12h 후성활솔위75%，현저고우기타각실험조(P<0.05)。채용실시형광정량PCR방법연구료불동간로협박조건대척미백하혈세포화간이선조직중열휴극단백70(HSP70)화ferritin기인표체적영향。간로협박능유도척미백하혈세포、간이선 HSP70기인적표체상조，간이선중적고표체시간상대혈세포중출현적교조；간로협박능유도습윤저온P조척미백하혈세포화간이선ferritin기인적표체상조，병현저고우대조조(P<0.05)，이차각조직 ferritin 기인표체적상조시간구유차이성，혈세포최선상조。기여실험조척미백하각조직ferritin기인표체균하조，병현저저우대조조(P<0.05)。상술결과표명，재척미백하수간로협박적내수범위내， HSP70화ferritin기인발휘항양화공능。
The conventional acute test method was applied to detect the effect of dry exposure on survival rates in Exopalaemon carinicauda of different groups. The results showed that survival rate of group P was 75%after dry exposure for 12h, which was significantly higher than that of other groups (P<0.05). Quantitative real-time PCR was applied to de-tect the expression profiles of heat shock protein (HSP70) and ferritin gene in haemocytes and hepatopancreas of E. carinicauda after challenged by different dry exposure stress conditions. The results showed that Dry exposure stress could induce HSP70 gene expression in haemocytes and hepatopancreas of E. carinicauda, and HSP70 gene expression up-regulating time in hepatopancreas was significantly earlier than that in haemocytes (P<0.05). Dry exposure stress could induce fetrritin gene expression of group P (wet and low temperature) in blood cells and hepatopancreas of E. carinicauda, which was significantly higher than that of control group, and fetrritin gene expression up-regulating time in blood cells was significantly earlier than that in hepatopancreas (P<0.05). The expression levels of ferritin gene in other groups were significantly down-regulated, significantly lower than that of control group P (P<0.05). These results suggested that HSP70 and ferritin gene play important roles under dry exposure stresses by serving as antioxidant in E. carinicauda.