CAJ | 학술논문

　　采用不同饲料配方投喂，研究了普通豆粕(对照组)、发酵豆粕和活性菌肽豆蛋白配合饲料对鲫(Carassius auratus)生长性状影响的研究。结果表明，活性菌肽豆蛋白组的相对体重生长率高于对照组41.87%(P<0.01)和发酵豆粕组19.11%；血清中的三碘甲状腺原氨酸(T3)、甲状腺素(T4)、胰岛素样生长因子-I(IGF-I)和生长激素(GH)含量活性菌肽豆蛋白组最高，其次是发酵豆粕组，而对照组最低；实时定量 PCR检测显示： IGFs(IGF-I和 IGF-II)基因的表达量在活性菌肽豆蛋白组中最高，其次是发酵豆粕组，对照组的表达量最低。GHR基因的表达量在活性菌肽豆蛋白组和发酵豆粕组间无显著性差异，但明显高于对照组。因此，活性菌肽豆蛋白可能通过调控鲫血清GH、IGF-I、T3和T4等激素水平，及肝脏GHR和IGFs基因的表达，进而影响鲫的生长。
　　채용불동사료배방투위，연구료보통두박(대조조)、발효두박화활성균태두단백배합사료대즉(Carassius auratus)생장성상영향적연구。결과표명，활성균태두단백조적상대체중생장솔고우대조조41.87%(P<0.01)화발효두박조19.11%；혈청중적삼전갑상선원안산(T3)、갑상선소(T4)、이도소양생장인자-I(IGF-I)화생장격소(GH)함량활성균태두단백조최고，기차시발효두박조，이대조조최저；실시정량 PCR검측현시： IGFs(IGF-I화 IGF-II)기인적표체량재활성균태두단백조중최고，기차시발효두박조，대조조적표체량최저。GHR기인적표체량재활성균태두단백조화발효두박조간무현저성차이，단명현고우대조조。인차，활성균태두단백가능통과조공즉혈청GH、IGF-I、T3화T4등격소수평，급간장GHR화IGFs기인적표체，진이영향즉적생장。
Using different feeding formulas, the effects of common soybean, fermented soybean and active microbial peptide soybean meals on growth appearance of crucian carp Carassius auratus were studied. The results indicat that the relative body weight growth rate was higher in active microbial peptide soybean group (41.87%) than control and fer-mented soybean group (19.11%higher);the serum triiodothyronine (T3), thyroxine (T4), insulin like growth factor I (IGF-I) and growth hormone (GH) levels of active microbial peptide soybean group were the highest with following fermented soybean group and the lowest in control group. Realtime-PCR indicated that IGFs (IGF-I and IGF-II) gene expressions were the highest in active microbial peptide soybean group, lower in fermented soybean group and the lowest in control group; GHR showed no significant difference between fermented soybean group and active microbial peptide soybean group, but both two groups were higher than control group. Thus, active microbial peptide soybean may regulate GH, IGF-I, T3 and T4 in serum as well as GHR and IGFs gene expression in liver to affect growth in C. auratus.