CAJ | 학술논문

　　采用酵母双杂交技术，对草鱼呼肠孤病毒096(GCRV096)非结构蛋白NS38相互作用的蛋白进行了筛选，并对阳性克隆中的插入序列进行测序与生物信息学分析。结果表明，含有重组质粒pGBKT7-NS38的酵母菌Y2HGold与含有草鱼肾脏细胞(CIK)cDNA文库质粒的酵母菌Y187融合成功，并筛选得到3株阳性克隆；发现两条不同的插入序列，其中一条序列编码的蛋白与40S核糖体蛋白 S16亚基的同源性高达99%，而另一序列相应的蛋白不存在匹配的蛋白。该结果为进一步深入研究NS38蛋白在GCRV096复制过程中的生物学功能奠定了基础。
　　채용효모쌍잡교기술，대초어호장고병독096(GCRV096)비결구단백NS38상호작용적단백진행료사선，병대양성극륭중적삽입서렬진행측서여생물신식학분석。결과표명，함유중조질립pGBKT7-NS38적효모균Y2HGold여함유초어신장세포(CIK)cDNA문고질립적효모균Y187융합성공，병사선득도3주양성극륭；발현량조불동적삽입서렬，기중일조서렬편마적단백여40S핵당체단백 S16아기적동원성고체99%，이령일서렬상응적단백불존재필배적단백。해결과위진일보심입연구NS38단백재GCRV096복제과정중적생물학공능전정료기출。
The proteins interactive with non-structural protein NS38 of GCRV096 (grass carp reovirus) was screened in a yeast two-hybrid system, and the inserted fragments in positive clones were sequenced and analyzed bioinformatically. The results show that yeast Y2HGold containing the recombinant vector of NS38 gene was successfully fused with yeast Y187 that pre-transformed with cDNA library plasmid of CIK. Three positive clones were obtained, and two inserted cDNA sequences were identified. The protein encoded by a cDNA fragment has higher homology of 99%with S16 subunit of 40S ribosomal protein. These results may provide a basis for further study on the biological function of NS38 in the duplication of GCRV096.