CAJ | 학술논문

　　利用单壁碳纳米管(SWNTs)的荧光淬灭作用制备了核酸适体荧光传感器并用于汞离子的检测分析。其基本原理是：Hg2+的引入使核酸适体由单链转化为双链，双链嵌入剂SYBR Green 1（SG）嵌入双链DNA堆积碱基之间，产生强烈的荧光信号，SWNTs的荧光淬灭作用可以提高传感器的信背比。该传感器能定量检测Hg2+，在200 nmol/L~3.00μmol/L浓度范围内呈线性关系，其检测下限为139 nmol/L。该传感器提供了一种简便、有效并且能广泛应用的汞离子检测平台。
　　이용단벽탄납미관(SWNTs)적형광쉬멸작용제비료핵산괄체형광전감기병용우홍리자적검측분석。기기본원리시：Hg2+적인입사핵산괄체유단련전화위쌍련，쌍련감입제SYBR Green 1（SG）감입쌍련DNA퇴적감기지간，산생강렬적형광신호，SWNTs적형광쉬멸작용가이제고전감기적신배비。해전감기능정량검측Hg2+，재200 nmol/L~3.00μmol/L농도범위내정선성관계，기검측하한위139 nmol/L。해전감기제공료일충간편、유효병차능엄범응용적홍리자검측평태。
In this study, single-walled carbon nanotubes (SWNTs) are applied in a fluorescence biosensor for mer-cury ion analysis. The basic principle is that the conformation of the T-rich single-strand aptamer changes to a dou-ble-strand owning to the specific binding between Hg2+and thymine–thymine (T–T) base pair. The SYBR Green 1 (SG) inserts into the double strand aptamer with a strong fluorescence signal. The SWNTs produces a good signal-to-background ratio. The linear range of proposed sensor for mercury ion quantitative determination is from 200 nmol/L~3.00μmol/L with a detection limit of 139 nmol/L. This sensor provides a simple, effective, and universal platform for mercury ion detection.