华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2013年
2期
225-230
,共6页
谢凤行%张峰峰%周可%赵玉洁
謝鳳行%張峰峰%週可%趙玉潔
사봉행%장봉봉%주가%조옥길
解淀粉芽孢杆菌%发酵参数%优化
解澱粉芽孢桿菌%髮酵參數%優化
해정분아포간균%발효삼수%우화
Bacillus amyloliquefaciens%Fermentation parameters%Optimization
采用单因素试验和正交试验对具有水质净化功能的解淀粉芽孢杆菌HN的培养基成分及培养条件进行了优化,并采用100 L发酵罐对优化发酵技术参数进行了验证试验。结果表明,优化的培养基成分为:蛋白胨2.0 g/L,牛肉膏3.0 g/L,可溶性淀粉7.0 g/L,氯化钠6.0 g/L;培养条件为:培养基的初始pH值为7.0,装液量为100 mL/250 mL,接种量为3%,温度为37℃,发酵周期24~30 h;在上述优化培养条件下的生长曲线结果显示,菌株HN在9 h后进入对数生长期,24 h达到稳定期,菌液浓度达35.6×108 cfu/mL,最高芽孢浓度达到30.4×108 cfu/mL;100 L发酵罐验证试验表明,37℃发酵26 h活菌浓度可达37.0×108 cfu/mL,芽孢浓度为33.6×108 cfu/mL。
採用單因素試驗和正交試驗對具有水質淨化功能的解澱粉芽孢桿菌HN的培養基成分及培養條件進行瞭優化,併採用100 L髮酵罐對優化髮酵技術參數進行瞭驗證試驗。結果錶明,優化的培養基成分為:蛋白胨2.0 g/L,牛肉膏3.0 g/L,可溶性澱粉7.0 g/L,氯化鈉6.0 g/L;培養條件為:培養基的初始pH值為7.0,裝液量為100 mL/250 mL,接種量為3%,溫度為37℃,髮酵週期24~30 h;在上述優化培養條件下的生長麯線結果顯示,菌株HN在9 h後進入對數生長期,24 h達到穩定期,菌液濃度達35.6×108 cfu/mL,最高芽孢濃度達到30.4×108 cfu/mL;100 L髮酵罐驗證試驗錶明,37℃髮酵26 h活菌濃度可達37.0×108 cfu/mL,芽孢濃度為33.6×108 cfu/mL。
채용단인소시험화정교시험대구유수질정화공능적해정분아포간균HN적배양기성분급배양조건진행료우화,병채용100 L발효관대우화발효기술삼수진행료험증시험。결과표명,우화적배양기성분위:단백동2.0 g/L,우육고3.0 g/L,가용성정분7.0 g/L,록화납6.0 g/L;배양조건위:배양기적초시pH치위7.0,장액량위100 mL/250 mL,접충량위3%,온도위37℃,발효주기24~30 h;재상술우화배양조건하적생장곡선결과현시,균주HN재9 h후진입대수생장기,24 h체도은정기,균액농도체35.6×108 cfu/mL,최고아포농도체도30.4×108 cfu/mL;100 L발효관험증시험표명,37℃발효26 h활균농도가체37.0×108 cfu/mL,아포농도위33.6×108 cfu/mL。
The single factor test and orthogonal-design experiment were applied to screen optimal fermentation technology such as medium composition and fermentation conditions for Bacillus amyloliquefaciens HN with water purification,and the fermentation parameters in fermentor of 100 L was scaled up successfully.The results showed that the strain HN was easy to cultivate in a medium with 2.0 g/L peptone,3.0 g/L beef extract,7.0 g/L soluble starch,6.0 g/L NaCl and the optimum fermentation conditions were initial culture medium pH 7.0,inoculation rate 3%(V/V),100 mL/250 mL medium volume at 37 ℃for 24-30 h.Under this condition,strain HN reached loga-rithmic phase at 9 h,came to stable period after cultured at 37 ℃for 24 h and the bacteria concentration reached 35.6 ×108 cfu/mL and the spore concentration was up to 30.4 ×10 8 cfu/mL in 250 mL triangular flask.The valida-tion test in 100 L fermentor result indicated that bacteria number and spore concentration reached 37.0 ×10 cfu/mL and 33.6 ×10 8 cfu/mL ,respectively after fermented at 37 ℃for 26 h.