CAJ | 학술논문

　　以青菜为材料，研究使用不同浓度N-苯基-2-萘胺处理后对青菜生长及其体内AsA-GSH循环的影响。结果表明，使用不同浓度N-苯基-2-萘胺对青菜的株高及生物量产生不同的影响，其中以0．50 mg/L的处理效果最好，当浓度大于0．50 mg/L时，株高和生物量均随着N-苯基-2-萘胺使用浓度的增加而降低；处理后还原型抗坏血酸（AsA）、脱氢抗坏血酸（DHA）、总量Vc和AsA/DHA比值的变化趋势相似，0．50 mg/L处理下，AsA、DHA、总量Vc、AsA/DHA比值与对照相比均显著增加，当浓度大于0．50 mg/L时，AsA、DHA、总量Vc、AsA/DHA比值与对照相比均显著降低；在AsA循环中的抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）和单脱氢抗坏血酸还原酶（MDHAR）活性变化趋势相似，均随着处理浓度增加呈现出先增加后降低的趋势，其中以0．50 mg/L处理下酶活性最高，而抗坏血酸氧化酶（AAO）活性则随着处理浓度的增加而逐渐降低，以0．25 mg/L处理效果最好；在还原型谷胱甘肽（GSH）循环中，当处理浓度小于2．50 mg/L时，氧化型谷胱甘肽（GSSG）含量随着处理浓度的增加与对照相比稍有下降，但差异不显著，GSH含量与谷胱甘肽还原酶（GR）活性变化趋势相似，随着处理浓度增加呈现出先增加而后逐渐降低，其中以0．50 mg/L处理下效果最好。说明合理利用水葫芦中化感物质，有助于植株的生长，同时增加了体内AsA-GSH代谢循环，提高了青菜的抗氧化防御能力。
　　이청채위재료，연구사용불동농도N-분기-2-내알처리후대청채생장급기체내AsA-GSH순배적영향。결과표명，사용불동농도N-분기-2-내알대청채적주고급생물양산생불동적영향，기중이0．50 mg/L적처리효과최호，당농도대우0．50 mg/L시，주고화생물량균수착N-분기-2-내알사용농도적증가이강저；처리후환원형항배혈산（AsA）、탈경항배혈산（DHA）、총량Vc화AsA/DHA비치적변화추세상사，0．50 mg/L처리하，AsA、DHA、총량Vc、AsA/DHA비치여대조상비균현저증가，당농도대우0．50 mg/L시，AsA、DHA、총량Vc、AsA/DHA비치여대조상비균현저강저；재AsA순배중적항배혈산과양화물매（APX）、탈경항배혈산환원매（DHAR）화단탈경항배혈산환원매（MDHAR）활성변화추세상사，균수착처리농도증가정현출선증가후강저적추세，기중이0．50 mg/L처리하매활성최고，이항배혈산양화매（AAO）활성칙수착처리농도적증가이축점강저，이0．25 mg/L처리효과최호；재환원형곡광감태（GSH）순배중，당처리농도소우2．50 mg/L시，양화형곡광감태（GSSG）함량수착처리농도적증가여대조상비초유하강，단차이불현저，GSH함량여곡광감태환원매（GR）활성변화추세상사，수착처리농도증가정현출선증가이후축점강저，기중이0．50 mg/L처리하효과최호。설명합리이용수호호중화감물질，유조우식주적생장，동시증가료체내AsA-GSH대사순배，제고료청채적항양화방어능력。
Effects of N-phenyl-2-naphthylamine on the growth and AsA-GSH circulation metabolism in Chinese cabbage were studied in the paper.The results showed that the different phenomena were found in the shoot height and biomass when the Chinese cabbages were treated by the different N-phenyl-2-naphthylamine concentrations .Es-pecially,the treatment of 0.50 mg/L had the best effect.The shoot height and fresh weight significantly increased compared with the control in the different sampling periods .However,when the concentration of N-phenyl-2-naphth-ylamine was more 0.50 mg/L,the shoot height and biomass remarkably decreased with the increase of N-phenyl-2-naphthylamine concentration.The contents of reduced ascorbate (AsA),dehydroascorbate(DHA),total Vc and ratio of AsA/DHA had the same trends.In the treatments of 0.50 mg/L,contents of AsA,DHA,total Vc and ratio of AsA/DHA significantly increased compared with the control .When the concentration of N-phenyl-2-naphthylamine was more 0.50 mg/L,the contents of total Vc,AsA,DHA and ratio of AsA/DHA remarkably decreased with the in-crease of N-phenyl-2-naphthylamine concentration .In the AsA cycle, the activities of ascorbic acid peroxidase (APX),dehydroascorbate reductase(DHAR)and monodehydroascorbate reductase (MDHAR) had the same change trends.All the activities showed the increase firstly and then showed the decrease with the increase of N -phenyl-2-naphthylamine concentration,especially,in the treatment of 0.50 mg/L,whereas the ascorbate oxidase(AAO) pres-ented the gradually decrease trend with the increase of N-phenyl-2-naphthylamine concentration.The treatment of 0.25 mg/L had the best effect about the AAO activity .The content of oxidized glutathione (GSSG) showed a little decrease compared with the control in the GSH cycle when the concentration was lower than 2.50 mg/L.It showed no difference compared with the control .But the reduced glutathione (GSH) content and the glutathione reductase (GR) appeared the increase firstly and then the decrease with the increase of N-phenyl-2-naphthylamine concentra-tion.The treatment of 0.50 mg/L had the best effect.All the results suggested that to reasonable use the allelochem-icals in Water hyacinth could promote the growth ,enhance AsA-GSH circulation metabolism and maintain higher antioxidative stress capacity in the Chinese cabbage .