CAJ | 학술논문

　　以蓖麻茎秆为试材，研究了影响cDNA-AFLP标记蓖麻差异基因反应体系的几个关键因素，建立了适宜蓖麻的cDNA-AFLP分析体系。结果表明：当裂解液为1．4 mL时提取的RNA浓度最高，EcoRⅠ和MseⅠ的组合6 h可将ds-cDNA酶切完全，与相应接头连接过夜后产物用于预扩增，预扩增产物稀释15倍时作为选择性扩增模板可以获得条带丰富且重复性好的结果。蓖麻cDNA-AFLP反应体系的建立为进一步研究蓖麻株高、叶型等相关基因奠定了基础。
　　이비마경간위시재，연구료영향cDNA-AFLP표기비마차이기인반응체계적궤개관건인소，건립료괄의비마적cDNA-AFLP분석체계。결과표명：당렬해액위1．4 mL시제취적RNA농도최고，EcoRⅠ화MseⅠ적조합6 h가장ds-cDNA매절완전，여상응접두련접과야후산물용우예확증，예확증산물희석15배시작위선택성확증모판가이획득조대봉부차중복성호적결과。비마cDNA-AFLP반응체계적건립위진일보연구비마주고、협형등상관기인전정료기출。
In this paper,we studied the factors affecting cDNA-AFLP marked castor differential gene ,estab-lished the suitable cDNA-AFLP analysis system for caster.The results indicated that the concentration of the RNA can be the highest when the lysate volume was 1.4 mL.The enzyme combination EcoRⅠand MseⅠdigested ds-cDNA thoroughly for 6 h.Then it was linked with the connection and the product was used for pre-amplified.The re-producible result and the rich bands can be obtained that the pre-amplified product was diluted 15-fold then was se-lective amplified.The research could provide important evidence for further studying the gene of caster on plant height,leaf type and so on.