CAJ | 학술논문

　　以冀豆12为母本，冀nf 58为父本通过杂交构建包含175个家系的F9：11重组自交系群体为试验材料。采用134个SSR标记，利用Kosambi函数绘制了一张包含117个标记，长度为1373．6 cM的遗传图谱。运用QTL Cartogra-pher v2．5软件的复合区间作图法（CIM）和QTL Network 2．0软件的基于混合线性模型的区间作图法（NWIM）同时检测到2个控制开花期性状的QTL。其中，qFTO-1定位在O连锁群标记区间Satt581～Sat＿190内，加性效应值分别为2．49和2．02，增效基因来自nf 58，贡献率最高为27．2％；qFTC2-1定位在C2连锁群标记区间Satt557～Sat＿251内，加性效应值分别为-3．50和-3．84，增效基因来自冀豆12，贡献率最高为55．6％。2个连锁群上QTL间存在加性×加性上位互作效应。效应值为-0．6728，重组型大于亲本型。2个QTL对产量相关性状有显著影响，与表型相关分析结果的趋势一致。其中，qFTO-1平均提高单株产量2．62 g，qFTC2-1平均提高单株产量2．26 g。这些研究为分子标记辅助育种及分子克隆提供理论依据。
　　이기두12위모본，기nf 58위부본통과잡교구건포함175개가계적F9：11중조자교계군체위시험재료。채용134개SSR표기，이용Kosambi함수회제료일장포함117개표기，장도위1373．6 cM적유전도보。운용QTL Cartogra-pher v2．5연건적복합구간작도법（CIM）화QTL Network 2．0연건적기우혼합선성모형적구간작도법（NWIM）동시검측도2개공제개화기성상적QTL。기중，qFTO-1정위재O련쇄군표기구간Satt581～Sat＿190내，가성효응치분별위2．49화2．02，증효기인래자nf 58，공헌솔최고위27．2％；qFTC2-1정위재C2련쇄군표기구간Satt557～Sat＿251내，가성효응치분별위-3．50화-3．84，증효기인래자기두12，공헌솔최고위55．6％。2개련쇄군상QTL간존재가성×가성상위호작효응。효응치위-0．6728，중조형대우친본형。2개QTL대산량상관성상유현저영향，여표형상관분석결과적추세일치。기중，qFTO-1평균제고단주산량2．62 g，qFTC2-1평균제고단주산량2．26 g。저사연구위분자표기보조육충급분자극륭제공이론의거。
A cross of Jidou 12 and nf 58 was made to construct a recombinant inbred line of F 9:11 population which has 175 progenies as the experimental material .A genetic linkage map of soybean genome was constructed , which include 117 markers and covering 1 373.6 cM.CIM method of QTL Cartographer v2.5 software and NWIM method of QTL Network 2.0 were used to identify quantitative trait loci and two QTLs were detected .qFTO-1 were located in the marker interval Satt581~Sat_190 of O linkage group,additive value were 2.49 and 2.02,favorable gene from nf 58 and the highest genetic contribution rate is27 .2 %.qFTC2-1 was located in the marker interval Satt557~Sat_251 of C2 linkage group,additive value were -3.50 and-3.84,favorable gene from Jidou 12 and the highest genetic contribution rate is 55.6%.Additive ×Additive epistatic effect was existent between the two QTLs . The value is -0.672 8 and the recombinant type is better than the parent .The two QTL effected on yield related traits obviously and consistent to the result of phenotype analysis .qFTO-1 can increase 2.62 g of yield per plant and qFTC2-1 can increase 2.26 g.These studies provide a theoretical basis for marker-assisted breeding and molecular cloning.