CAJ | 학술논문

　　采用水培的方法，研究4℃低温胁迫0，24，48，96 h后，外源NO供体硝普钠（SNP）对小麦幼苗生长、可溶性总糖含量、可溶性糖各组分含量及果聚糖合成酶（FBEs）等的影响，并采用qRT-PCR方法测定小麦幼叶中FBEs基因mRNA水平的变化。结果表明，外源NO促进小麦幼苗生长，提高低温胁迫下小麦幼叶果糖、葡萄糖、低聚果糖（DP3）、可溶性总糖等含量和果聚糖：果聚糖-1-果糖基转移酶（FFT）活性，并且在mRNA水平上调节FBEs基因表达。外源NO能够通过调控FBEs基因表达，进而影响果聚糖合成酶活性，促进果聚糖积累，提高小麦抵抗低温的能力。
　　채용수배적방법，연구4℃저온협박0，24，48，96 h후，외원NO공체초보납（SNP）대소맥유묘생장、가용성총당함량、가용성당각조분함량급과취당합성매（FBEs）등적영향，병채용qRT-PCR방법측정소맥유협중FBEs기인mRNA수평적변화。결과표명，외원NO촉진소맥유묘생장，제고저온협박하소맥유협과당、포도당、저취과당（DP3）、가용성총당등함량화과취당：과취당-1-과당기전이매（FFT）활성，병차재mRNA수평상조절FBEs기인표체。외원NO능구통과조공FBEs기인표체，진이영향과취당합성매활성，촉진과취당적루，제고소맥저항저온적능력。
The effects of exogenous NO on wheat (Triticum aestivum L.) growth,solubility sugar,fructan bio-synthesis metabolism etc.under 4 ℃chilling stress were investigated in seedlings ,subjected to different concentra-tions of sodium nitroprussiate(SNP,as nitric oxide donor) for 0,24,48,96 h.The results showed that,under chilling stress,exogenous NO could induce wheat weight ,fructose,glucose,low DP fructan,total soluble sugar content and fructan:fructan 1-fructosyltransferase(FFT) activity.Moreover,NO could regulate the gene expression at this level in following ways:sucrose:sucrose 1-fructosyltransferase (1-SST) and sucrose:fructan 6-fructosyltransferase (6-SFT) gene expression were inhibited by high concentration of SNP while 1-FFT was induced.Our report owing that exogenous NO alleviated the negative effects of non-freezing low temperatures by regulating FEBs and accumulating fructans.