CAJ | 학술논문

　　在研究珊瑚微生物混合共培养时发现：在混合培养中南海珊瑚内生细菌L-4对真菌的次生代谢产物明显产生影响，甚至产生此二微生物单独培养时所不产生的物质。对L-4发酵液进行次生代谢产物研究，从中分离纯化得到8个单体化合物，通过现代波谱分析（MS、 NMR等）和物理常数对照等手段确定其结构依次为：环（色-丙）二肽（1）、环（甘-酪）二肽（2）、环（丙-酪）二肽（3）、环（缬-酪）二肽（4）、环（甘-苯丙）二肽（5）、环（甘-脯）二肽（6）、环（丙-缬）二肽（7）、环（甘-丙）二肽（8）均为环二肽类物质，且均首次从南海珊瑚内生细菌中分离得到，对进一步研究其可能的代谢机制具有重要意义。
　　재연구산호미생물혼합공배양시발현：재혼합배양중남해산호내생세균L-4대진균적차생대사산물명현산생영향，심지산생차이미생물단독배양시소불산생적물질。대L-4발효액진행차생대사산물연구，종중분리순화득도8개단체화합물，통과현대파보분석（MS、 NMR등）화물리상수대조등수단학정기결구의차위：배（색-병）이태（1）、배（감-락）이태（2）、배（병-락）이태（3）、배（힐-락）이태（4）、배（감-분병）이태（5）、배（감-포）이태（6）、배（병-힐）이태（7）、배（감-병）이태（8）균위배이태류물질，차균수차종남해산호내생세균중분리득도，대진일보연구기가능적대사궤제구유중요의의。
The influence of secondary metabolites on the mix-cultivate marine bacteria and fungus were studied.The result indicated that bacteria obviously affected the secondary metabolites of fungus in the mix-cultivate, and even produce substances that were not found in the fungus or bacteria separately cul -ture by TLC.Eight compounds were obtained from the fermentation broth of coral-derived bacteria L-4 in South China Sea.Their structures were determined to be cyclo (Trp-Ala) (1), cyclo (Gly-Tyr) (2), cyclo (Ala-Tyr) (3), and cyclo (Val-Tyr) (4), cyclo (Gly-Phe) (5), cyclo (Gly-Pro) (6), cyclo (Ala-Val) (7), and cyclo (Gly-Ala) (8) by spectroscopic analysis.The compounds 1, 2, 3, 4, 5, 6, 7, 8 were all cyclo-dipeptides and first obtained from the marine coral-derived bacteri-um of South China Sea, which attributed to further research metabolism of mix-cultivation.