CAJ | 학술논문

　　为了检测猪繁殖与呼吸综合征病毒（Reproductive and respiratory syndrome virus，PRRSV）接种莱芜黑猪后病毒在体内的增殖分布规律，将15头 PRRSV 阴性的莱芜黑猪仔猪随机分成两组，试验组10头，对照组5头。分别于攻毒后3、7、14、21、28 d 各剖杀试验组2头，对照组1头，采集脾脏、肺脏、心脏、肝脏、肾脏、扁桃体、颌下淋巴结、腹股沟淋巴结和肠系膜淋巴结等组织，并应用建立的荧光定量 RT-PCR 方法对各组织中病毒进行定量。结果表明，PRRSV 攻毒后 d 3感染组猪的各组织中均检测到低拷贝病毒核酸存在，攻毒后7~21 d，各种组织中的病毒载量维持在较高水平，而攻毒后 d 28时，多数组织中的 PRRSV 含量明显下降。扁桃体及各种淋巴结中的病毒载量要明显高于其他脏器，显示 PRRSV 在感染莱芜黑猪后对淋巴组织具有组织嗜性。
　　위료검측저번식여호흡종합정병독（Reproductive and respiratory syndrome virus，PRRSV）접충래무흑저후병독재체내적증식분포규률，장15두 PRRSV 음성적래무흑저자저수궤분성량조，시험조10두，대조조5두。분별우공독후3、7、14、21、28 d 각부살시험조2두，대조조1두，채집비장、폐장、심장、간장、신장、편도체、합하림파결、복고구림파결화장계막림파결등조직，병응용건립적형광정량 RT-PCR 방법대각조직중병독진행정량。결과표명，PRRSV 공독후 d 3감염조저적각조직중균검측도저고패병독핵산존재，공독후7~21 d，각충조직중적병독재량유지재교고수평，이공독후 d 28시，다수조직중적 PRRSV 함량명현하강。편도체급각충림파결중적병독재량요명현고우기타장기，현시 PRRSV 재감염래무흑저후대림파조직구유조직기성。
In order to detect distribution of reproductive and respiratory syndrome viruses (PRRSV) in infected Laiwu black pigs, 10 pigs were infected with PRRSV and 5 pigs were used as non-infected controls. Two infected pigs and one uninfected pig were sacrificed at 3, 7, 14, 21 and 28 days post infection (DAI). Hearts, livers, spleens, lungs, kidneys, tonsils, submandibular lymph nodes, inguinal lymph nodes and mesenteric lymph nodes were collected, and viral loads were measured in fluorescence quantitative RT-PCR. The viral copies of PRRSV were detected at 3 DAI, increased to high level at 7 to 21 DAI, and decreased significantly at 28 DAI. The viral copies of PRRSV in tonsils and lymph nodes were significantly higher than that in other tissues, which indicated a distinct lymph tissue tropism in Laiwu black pigs.