CAJ | 학술논문

　　为研究 UL14蛋白在马立克氏病病毒感染过程中的生物学作用，本文以 RB1B 毒株感染的鸡胚成纤维细胞基因组 DNA为模板，经 PCR 扩增出 UL14基因，然后克隆入 pET32a 载体，转化 BL21（DE3）工程细菌且经 IPTG 诱导进行原核表达。结果显示，pET32a-UL14重组质粒在大肠杆菌中表达，表达的重组蛋白具有可溶性。IFA 分析证实，用此融合蛋白免疫 BALB/c小鼠后获得的多抗血清能与 MDV RB1B 感染的 CEF 细胞发生特异性反应。本研究结果为 UL14蛋白的生物学功能研究提供了条件。
　　위연구 UL14단백재마립극씨병병독감염과정중적생물학작용，본문이 RB1B 독주감염적계배성섬유세포기인조 DNA위모판，경 PCR 확증출 UL14기인，연후극륭입 pET32a 재체，전화 BL21（DE3）공정세균차경 IPTG 유도진행원핵표체。결과현시，pET32a-UL14중조질립재대장간균중표체，표체적중조단백구유가용성。IFA 분석증실，용차융합단백면역 BALB/c소서후획득적다항혈청능여 MDV RB1B 감염적 CEF 세포발생특이성반응。본연구결과위 UL14단백적생물학공능연구제공료조건。
To investigate the biological function of UL14 protein during the course of MDV infection, UL14 gene from CEF cells infected with RB1B strain was amplified by PCR. UL14 gene was cloned into pET32a (+) vector and expressed in E.coli BL21 (DE3). Recombinant His-UL14 protein expressed in E.coli was soluble. The BALB/c mice were immunized with recombinant UL14 protein and resulting antiserum was used in IFA. The specific inflorescence was visualized in CEF cells infected with MDV strain RB1B .