CAJ | 학술논문

　　从2011年开始，全国多个省份发生了新生仔猪疑似伪狂犬病的典型症状。2012年下半年，我们从江苏省某伪狂犬病疫苗免疫猪场中发病仔猪的脑组织中分离到一株伪狂犬病毒（Pseudorabies virus，PRV），并进行了部分生物学特性分析。从发病死亡仔猪脑组织中，利用 PCR 技术针对 PRV 的 gB 基因和 gE 基因分别扩增出了两条特异性片段，经序列分析初步确定为伪狂犬病毒感染。随后，将病料上清接种 Vero 细胞，24 h 后细胞发生变圆、空泡、合胞体等典型的细胞病变，将分离的病毒经过6轮蚀斑纯化后，用 PRV 抗体 IFA 检测显示，感染细胞产生阳性荧光反应。分离的病毒在 Vero 细胞上的生长滴度可以达到107.43 TCID50/mL。将病毒接种小鼠很快出现了奇痒并最终死亡等伪狂犬症状，且比经典的 PRV（S 株）对小鼠的 LD50低。对gE 毒力基因序列的比对分析表明，该毒株与经典 PRV 强毒和疫苗株有明显差异。
　　종2011년개시，전국다개성빈발생료신생자저의사위광견병적전형증상。2012년하반년，아문종강소성모위광견병역묘면역저장중발병자저적뇌조직중분리도일주위광견병독（Pseudorabies virus，PRV），병진행료부분생물학특성분석。종발병사망자저뇌조직중，이용 PCR 기술침대 PRV 적 gB 기인화 gE 기인분별확증출료량조특이성편단，경서렬분석초보학정위위광견병독감염。수후，장병료상청접충 Vero 세포，24 h 후세포발생변원、공포、합포체등전형적세포병변，장분리적병독경과6륜식반순화후，용 PRV 항체 IFA 검측현시，감염세포산생양성형광반응。분리적병독재 Vero 세포상적생장적도가이체도107.43 TCID50/mL。장병독접충소서흔쾌출현료기양병최종사망등위광견증상，차비경전적 PRV（S 주）대소서적 LD50저。대gE 독력기인서렬적비대분석표명，해독주여경전 PRV 강독화역묘주유명현차이。
The newborn pigs in many herds have suffered from a disease that is clinically similar to pseudorabies in China since 2011. In late 2012, the disease outbreak occurred in a herd in Jiangsu province that received PRV vaccination. Brain tissue of a dead piglet was collected and examined for PRV by PCR. Two pairs of primers specific for PRV gB and gE were designed and used to amplify the corresponding DNA segments. The resulting DNA segments were sequenced and shown to share very high homology with known PRV. A pseudorabies virus was isolated from this dead piglet. The homogenated brain tissue was inoculated to Vero cells. Classical cytopathogenic effects of herpesvirus appeared in Vero cells at 24 hours post-inoculation. The virus was cloned by six rounds of plaque purification in Vero cells. The infected cells showed positive reaction with PRV antibodies in IFA. Therefore, the isolated virus was confirmed to be PRV. The PRV isolate grew well in Vero cells with the titer up to 107.43 TCID50/mL. The mice infected with this isolate developed unbearable itching and died later. The LD50 of the isolate was lower than that of PRV S strain. Genetic analysis of gE gene revealed that the isolate was different from classical virulent strain and vaccine strain.