CAJ | 학술논문

　　以溶液浇注法制备丝素蛋白/聚乙二醇（PEG）混合薄膜，利用傅里叶红外光谱（FT-IR）和差示扫描量热（ DSC）分析薄膜材料结构的变化及对其力学性能和溶解性的影响，并以罗丹明B为模型药物构建药物缓释体系，分析罗丹明B从混合薄膜中释放的动力学，探讨利用PEG调节丝素蛋白材料的药物释放性能的可能性。结果显示，PEG与丝素蛋白具有良好的相容性，在作为增塑剂的同时引起丝素蛋白由无规卷曲向β-折叠结构的转变，可以在不同程度上调节混合薄膜的力学性能、耐水性和药物缓释性能。随着PEG含量的增加，丝素蛋白/PEG混合薄膜的拉伸强度和断裂延长率先增加后减小，分别于CPEG为10％和20％附近达到最大值，而在水中的溶失率的变化规律与拉伸强度刚好相反，在CPEG为10％附近达到最小值。与纯丝素蛋白薄膜相比，添加PEG极大延缓了药物模型分子罗丹明B的释放。罗丹明B的释放属于非典型Fickian扩散机理，混合薄膜中丝素的silkⅡ结晶和非结晶区域具有不同的药物释放特征，增加PEG添加量造成罗丹明B释放常数k的下降和扩散指数n的增加。
　　이용액요주법제비사소단백/취을이순（PEG）혼합박막，이용부리협홍외광보（FT-IR）화차시소묘량열（ DSC）분석박막재료결구적변화급대기역학성능화용해성적영향，병이라단명B위모형약물구건약물완석체계，분석라단명B종혼합박막중석방적동역학，탐토이용PEG조절사소단백재료적약물석방성능적가능성。결과현시，PEG여사소단백구유량호적상용성，재작위증소제적동시인기사소단백유무규권곡향β-절첩결구적전변，가이재불동정도상조절혼합박막적역학성능、내수성화약물완석성능。수착PEG함량적증가，사소단백/PEG혼합박막적랍신강도화단렬연장솔선증가후감소，분별우CPEG위10％화20％부근체도최대치，이재수중적용실솔적변화규률여랍신강도강호상반，재CPEG위10％부근체도최소치。여순사소단백박막상비，첨가PEG겁대연완료약물모형분자라단명B적석방。라단명B적석방속우비전형Fickian확산궤리，혼합박막중사소적silkⅡ결정화비결정구역구유불동적약물석방특정，증가PEG첨가량조성라단명B석방상수k적하강화확산지수n적증가。
Blend films were prepared from silk fibroin ( SF) and polyethylene glycol ( PEG) using a solution casting method , and the structural change of blend films were investigated by Fourier trans-form infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC).The results were utilized to reveal the influence of PEG addition on the mechanical and dissolution properties of the blend films and the release kinetics of Rhodamine B , a model compound loaded in the SF/PEG ma-trix, with the purpose to regulate the drug releasing property of SF biomaterial by blending with PEG.It was found that PEG was compatible with SF and capable of inducing conformational transi -tion of SF from random coil to β-sheet, resulting in changes in mechanical and dissolution proper-ties, as well as drug release feature of the blend films.With increasing PEG content , the tensile strength and elongation of blend films first increased and then decreased , reaching a maximum a-round 10%and 20%, respectively.On the contrast , the dissolution of blend films first decreased and then increased , reaching a minimum around 10%.Compared with the pure SF film , addition of PEG greatly reduced the release of Rhodamine B.It was suggested that Rhodamine B released from SF/PEG blend films via a non-typical Fickian diffusion mechanism , of which the release constant k de-creased and diffusion parameter n increased with increasing PEG content , maybe due to the different releasing feature of the silk II crystalline and noncrystalline region of SF .