CAJ | 학술논문

　　为了准确、快速检测食用油中苯并（a）芘的含量，建立了食用油中苯并（a）芘的超声波辅助-绿色溶剂萃取及GC/MS联用技术检测方法，其检出限和定量限分别为0.017μg/L和0.053μg/L，加标回收率大于95％，日内和日间相对标准偏差分别为1.32％～1.76％和2.46％～3.17％，表明该方法符合痕量分析要求。实验考察了萃取剂种类、配比、用量、超声波功率、温度和时间对苯并（a）芘萃取的影响，获得了较优的萃取工艺条件：ACN-DMC配比（V/V）7∶3，萃取剂用量25 mL，超声功率300 W，温度20℃，时间10 min/次，萃取两次。采用该方法测定38个食用油样中苯并（ a）芘含量，结果表明，其中30个不同品牌的新鲜食用油苯并（ a）芘含量范围为0.09～8.17μg/kg；8个油炸食用油苯并（ a）芘含量范围为6.03～11.73μg/kg。本方法耗时短且减少了有毒溶剂的使用，为食用油中含有的苯并（ a）芘分析检测提供了一种科学简捷的方法。
　　위료준학、쾌속검측식용유중분병（a）비적함량，건립료식용유중분병（a）비적초성파보조-록색용제췌취급GC/MS련용기술검측방법，기검출한화정량한분별위0.017μg/L화0.053μg/L，가표회수솔대우95％，일내화일간상대표준편차분별위1.32％～1.76％화2.46％～3.17％，표명해방법부합흔량분석요구。실험고찰료췌취제충류、배비、용량、초성파공솔、온도화시간대분병（a）비췌취적영향，획득료교우적췌취공예조건：ACN-DMC배비（V/V）7∶3，췌취제용량25 mL，초성공솔300 W，온도20℃，시간10 min/차，췌취량차。채용해방법측정38개식용유양중분병（ a）비함량，결과표명，기중30개불동품패적신선식용유분병（ a）비함량범위위0.09～8.17μg/kg；8개유작식용유분병（ a）비함량범위위6.03～11.73μg/kg。본방법모시단차감소료유독용제적사용，위식용유중함유적분병（ a）비분석검측제공료일충과학간첩적방법。
To quickly and accurately analyze B (a)p in edible oils, an ultrasonic assisted extraction with environmental friendly solvent using gas chromatography-mass spectrometry ( GC-MS ) method was developed.The limits of detection and quantization obtained were 0.017μg/L and 0.053μg/L respectively , and the recovery rate was higher than 95% and the relative standard deviations were 1.32%~1.76% for inter-day and 2.46% ~3.17% for intra-day, demonstrating the proposed method was feasible and effective.Optimizing the conditions of ultrasound assisted extraction such as extractant, ration, dosage, ultrasonic power, temperature and time, an optimum method was deve-loped.The levels of B(a)p by using this method ranged from 0.09 μg/kg to 8.17 μg/kg in 30 flesh edible oil samples , from 6.03μg/kg to 11.73μg/kg in 8 frying oil samples .The development of this method provided a scientific and simple method for determination of B ( a) p in less time and solvent .