广西大学学报(自然科学版)
廣西大學學報(自然科學版)
엄서대학학보(자연과학판)
JOURNAL OF GUANGXI UNIVERSITY (NATURAL SCIENCE EDITION)
2013年
3期
527-531
,共5页
内部沸腾%银叶树叶%提取%总黄酮
內部沸騰%銀葉樹葉%提取%總黃酮
내부비등%은협수협%제취%총황동
inner ebullition%leaves of Heritiera littoralis%extraction%total flavonoids
为优化银叶树叶中总黄酮的内部沸腾法提取工艺,以提高提取物的黄酮产率及含量,采用单因素试验的方法,考察解吸及提取过程中的解吸剂浓度、解吸剂用量和解吸时间及提取剂浓度、提取温度、提取时间和提取剂用量等7个因素对提取效果的影响,结果表明,银叶树叶先用浓度80%乙醇3 mL/g解吸20 min,然后用浓度30%乙醇25 mL/g在85℃的条件下提取两次,每次5 min,在该工艺条件下总黄酮提取得率为13.5%,提取物的黄酮含量达到46.9%。与传统的醇提法及酶提法相比,内部沸腾法提取时间分别缩短了70 min和140 min,乙醇用量分别减少了46 mL和15 mL,但总黄酮提取率比前两种方法分别提高了4.8%和2.8%,黄酮含量分别提高了14.5%和3.1%。内部沸腾提取法快速、有效且经济,用于银叶树叶中总黄酮的提取效果良好。
為優化銀葉樹葉中總黃酮的內部沸騰法提取工藝,以提高提取物的黃酮產率及含量,採用單因素試驗的方法,攷察解吸及提取過程中的解吸劑濃度、解吸劑用量和解吸時間及提取劑濃度、提取溫度、提取時間和提取劑用量等7箇因素對提取效果的影響,結果錶明,銀葉樹葉先用濃度80%乙醇3 mL/g解吸20 min,然後用濃度30%乙醇25 mL/g在85℃的條件下提取兩次,每次5 min,在該工藝條件下總黃酮提取得率為13.5%,提取物的黃酮含量達到46.9%。與傳統的醇提法及酶提法相比,內部沸騰法提取時間分彆縮短瞭70 min和140 min,乙醇用量分彆減少瞭46 mL和15 mL,但總黃酮提取率比前兩種方法分彆提高瞭4.8%和2.8%,黃酮含量分彆提高瞭14.5%和3.1%。內部沸騰提取法快速、有效且經濟,用于銀葉樹葉中總黃酮的提取效果良好。
위우화은협수협중총황동적내부비등법제취공예,이제고제취물적황동산솔급함량,채용단인소시험적방법,고찰해흡급제취과정중적해흡제농도、해흡제용량화해흡시간급제취제농도、제취온도、제취시간화제취제용량등7개인소대제취효과적영향,결과표명,은협수협선용농도80%을순3 mL/g해흡20 min,연후용농도30%을순25 mL/g재85℃적조건하제취량차,매차5 min,재해공예조건하총황동제취득솔위13.5%,제취물적황동함량체도46.9%。여전통적순제법급매제법상비,내부비등법제취시간분별축단료70 min화140 min,을순용량분별감소료46 mL화15 mL,단총황동제취솔비전량충방법분별제고료4.8%화2.8%,황동함량분별제고료14.5%화3.1%。내부비등제취법쾌속、유효차경제,용우은협수협중총황동적제취효과량호。
In order to improve the yield and content of product and optimize the extraction technolo-gy of the total flavonoids in leaves of Heritiera littoralis by inner ebullition , single factor experiments were conducted to investigate the effects of seven factors on the extraction , including the concentra-tion and the amount of desorption agent , desorption time , the concentration and the amount of ex-tractant , extraction temperature and time .It was found that when leaves of Heritiera littoralis were desorbed 20 minutes using 80% ethanol 3 mL/g and then extracted twice ( 5 min/time ) in 30%ethanol 25 mL/g under 85 ℃, the yield of total flavonoids of 13.5%was obtained and the content of flavonoids in extractive reached 46.9%.Compared with the traditional ethanol and cellulase ex-traction methods , the inner ebullition extraction had the following obvious advantages that extraction time was reduced by 70 min and 140 min, ethanol consumption reduced by 46 mL and 15 mL, while the yields of total flavonoids increased by 4.8%and 2.8%, and the contents of flavonoids in extrac-tive raised by 14.5%and 3.1%,respectively .Inner ebullition extraction was rapid , effective and e-conomic in the extraction of total flavonoids from leaves of Heritiera littoralis.