CAJ | 학술논문

目的：研究抗HIV药物雷特格韦与利托那韦在大鼠原代肝细胞内联合用药的药代动力学，并分析其代谢相互作用。方法：大鼠原代肝细胞分离培养后，分别以雷特格韦及利托那韦不同时间给药。高效液相色谱法建立药物检测方法学，并以此分析细胞匀浆内药物浓度。结果：高效液相色谱测定方法学证明检测效果良好，药代动力学分析表明联合用药中两种药物在原代肝细胞内代谢基本不受影响。结论：雷特格韦与利托那韦之间没有药物代谢相互作用，能够在抗HIV临床治疗中联合使用。
목적：연구항HIV약물뢰특격위여리탁나위재대서원대간세포내연합용약적약대동역학，병분석기대사상호작용。방법：대서원대간세포분리배양후，분별이뢰특격위급리탁나위불동시간급약。고효액상색보법건립약물검측방법학，병이차분석세포균장내약물농도。결과：고효액상색보측정방법학증명검측효과량호，약대동역학분석표명연합용약중량충약물재원대간세포내대사기본불수영향。결론：뢰특격위여리탁나위지간몰유약물대사상호작용，능구재항HIV림상치료중연합사용。
Objective: To investigate the pharmacokinetics and relevant drug interaction of Raltegravir and Ritonavir in rat primary hepatocytes. Methods: Rat primary hepatocytes were cultured and treated with Raltegravir and/or Ritonavir for different times. Methodologies of HPLC analysis were constructed and used on the determination of drug concentrations in celllysis. Results: HPLC methodology was proved to be fairly accurate and reliable. The drug metabolisms of Raltegravir and Ritonavir were not interfered by each other. Conclusion: No drug interaction was detected between Raltegravir and Ritonavir, which are encouraged to be combine-used in clinic therapy on HIV infection.