神经损伤与功能重建
神經損傷與功能重建
신경손상여공능중건
NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION
2013年
3期
157-159
,共3页
闫秋月%李珍%胡文涛%方瑜%王淑楠%邱占东%张苏明
閆鞦月%李珍%鬍文濤%方瑜%王淑楠%邱佔東%張囌明
염추월%리진%호문도%방유%왕숙남%구점동%장소명
胰岛素样生长因子-1%诱导多潜能干细胞%细胞重编程
胰島素樣生長因子-1%誘導多潛能榦細胞%細胞重編程
이도소양생장인자-1%유도다잠능간세포%세포중편정
insulin-like growth factor-1%induced pluripotent stem cells%cellular reprogramming
目的：观察胰岛素样生长因子-1（IGF-1）对小鼠诱导多潜能干（iPS）细胞诱导效率的影响。方法：将鼠胚成纤维细胞分为对照组和实验组，2组均予经典四因子的逆转录病毒载体感染，实验组在培养基里加入IGF-1，进行iPS细胞诱导，并对其进行鉴定。结果：对照组和实验组的iPS细胞诱导效率分别为（0.05±0.0002）%和（0.01±0.0005）%，所获iPS细胞为阳性。结论：IGF-1提高小鼠iPS细胞的诱导效率。
目的：觀察胰島素樣生長因子-1（IGF-1）對小鼠誘導多潛能榦（iPS）細胞誘導效率的影響。方法：將鼠胚成纖維細胞分為對照組和實驗組，2組均予經典四因子的逆轉錄病毒載體感染，實驗組在培養基裏加入IGF-1，進行iPS細胞誘導，併對其進行鑒定。結果：對照組和實驗組的iPS細胞誘導效率分彆為（0.05±0.0002）%和（0.01±0.0005）%，所穫iPS細胞為暘性。結論：IGF-1提高小鼠iPS細胞的誘導效率。
목적：관찰이도소양생장인자-1（IGF-1）대소서유도다잠능간（iPS）세포유도효솔적영향。방법：장서배성섬유세포분위대조조화실험조，2조균여경전사인자적역전록병독재체감염，실험조재배양기리가입IGF-1，진행iPS세포유도，병대기진행감정。결과：대조조화실험조적iPS세포유도효솔분별위（0.05±0.0002）%화（0.01±0.0005）%，소획iPS세포위양성。결론：IGF-1제고소서iPS세포적유도효솔。
Objective:To study the effect of insulin-like growth factor-1 (IGF-1) on the induced efficiency of murine induced pluripotent stem(iPS) cells. Methods:Mouse embryonic fibroblasts were divided into control group and experimental group. The 2 groups were infected with retro-viruses expressing classic 4 factors while the medium of the experimental group was added IGF-1. Then the clones were determined. Result: The in-duced efficiency of the control group and the experimental group were (0.05± 0.0002)%and (0.01± 0.0005)%respectively. The clones were positive. Conclusion:IGF-1 improve the inducing efficiency of iPS cells.
