CAJ | 학술논문

　　微生物全细胞转化靛蓝的过程中，靛蓝易被游离细胞吸附，造成产物提取困难，是微生物产靛蓝工业化进程中面临的主要问题之一．为解决该问题，尝试了不同固定化方法将基因工程菌固定，其中海藻酸钠包埋法取得了最好的效果，最佳包埋条件为3．5％海藻酸钠、1％氯化钙，固定化细胞在有机-水两相体系中可以重复利用两次，首次转化6 h后，靛蓝浓度为15．9 mg/L ．另外，两相体系的引入有效避免了固定化细胞产靛蓝过程中伴随的产物分解现象，选择正十二烷作为有机相，优化得到其最佳比例为体积分数40％．体系对吲哚的最佳耐受浓度为400 mg/L ，在此条件下，靛蓝产率为0．28 mg/mg ．
　　미생물전세포전화전람적과정중，전람역피유리세포흡부，조성산물제취곤난，시미생물산전람공업화진정중면림적주요문제지일．위해결해문제，상시료불동고정화방법장기인공정균고정，기중해조산납포매법취득료최호적효과，최가포매조건위3．5％해조산납、1％록화개，고정화세포재유궤-수량상체계중가이중복이용량차，수차전화6 h후，전람농도위15．9 mg/L ．령외，량상체계적인입유효피면료고정화세포산전람과정중반수적산물분해현상，선택정십이완작위유궤상，우화득도기최가비례위체적분수40％．체계대신타적최가내수농도위400 mg/L ，재차조건하，전람산솔위0．28 mg/mg ．
The extraction of indigo is delayed due to the adsorption of indigo by the free whole cell , which is one of the major difficulties during the biotransformation of indigo .Three immobilization methods are used , and sodium alginate immobilization method obtains the best results . The immobilized cell can be repeatedly used for two times in a biphasic system under the optimum immobilization condition :3 .5% sodium alginate ,1% CaCl2 ,and the indigo production is 15 .9 mg/L after 6 h of the first transformation .Besides ,the indigo decomposition can be avoided by the biphasic system with 40% dodecane as the optimal organic phase .The optimum indole tolerance concentration is 400 mg/L ,and the productivity of indigo is 0 .28 mg/mg .