CAJ | 학술논문

　　目的从放线菌 Streptomyces sp SS 发酵液中分离新型活性尿苷肽类似物。方法发酵液经 D4006大孔吸附树脂、DEAE-葡聚糖凝胶及制备液相分离纯化，获得单一化合物；根据 UV、MS、MS/MS、1D 和2D NMR 确定化合物结构，并进行抗菌活性研究。结果分离纯化得到1个 N-末端含有1-甲基-6-羟基四氢异喹啉的尿苷肽类化合物 Sansanmycin P。初步体外抗菌活性，抗铜绿假单胞菌 MIC >32μg/ml，抗结核杆菌 MIC >32μg/ml。结论 Sansanmycin P 为全新结构 Sansanmycins 类似物，具有一定抗结核杆菌和铜绿假单胞菌活性。
　　목적종방선균 Streptomyces sp SS 발효액중분리신형활성뇨감태유사물。방법발효액경 D4006대공흡부수지、DEAE-포취당응효급제비액상분리순화，획득단일화합물；근거 UV、MS、MS/MS、1D 화2D NMR 학정화합물결구，병진행항균활성연구。결과분리순화득도1개 N-말단함유1-갑기-6-간기사경이규람적뇨감태류화합물 Sansanmycin P。초보체외항균활성，항동록가단포균 MIC >32μg/ml，항결핵간균 MIC >32μg/ml。결론 Sansanmycin P 위전신결구 Sansanmycins 유사물，구유일정항결핵간균화동록가단포균활성。
Objective To isolate new uridyl peptide components from the fermentation broth of Streptomyces sp. SS. Methods The new component was isolated and purified by various chromatography methods, such as macroporous adsorption resins, ion exchange resin and reversed-phase HPLC. Its structure was elucidated by spectroscopic analyses, such as UV, MS, MS/MS, 1D and 2D NMR.Results A novel uridyl peptide antibiotic, Sansanmycin P, with an N-terminal containing 1-methyl-6-hydroxy-1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid was obtained. Antibacterial activity in vitro, resistance of Pseudomonas aeruginosa MIC > 32 μg/ml, Mycobacterium tuberculosis MIC > 32 μg/ml. Conclusion Sansanmycin P is a novel Sansanmycin analogue with certain antimicrobial activities against Mycobacterium tuberculosis and Pseudomonas aeruginosa.