CAJ | 학술논문

　　目的研究芹菜素对人肺癌 A549细胞增殖抑制和凋亡诱导作用与 Bax、Bcl-2蛋白表达的影响。方法10~80μmol/L 不同浓度芹菜素作用 A549细胞，采用 MTT 法检测芹菜素对 A549细胞增殖抑制作用；Hoechst 33258细胞核染色法观察芹菜素诱导细胞凋亡形态学的变化；流式细胞仪 AnnexinV- FITC/PI 双染色法检测细胞凋亡率；Western blot 法检测凋亡相关蛋白 Bax 和 Bcl-2表达的变化。结果 MTT 法显示，芹菜素对 A549细胞有显著的增殖抑制作用（P <0.01），且具浓度和时间依赖性；荧光显微镜下观察到芹菜素处理组细胞出现典型的凋亡形态学改变：细胞核固缩、染色质凝集和核碎片化等；流式细胞仪分析结果显示，芹菜素呈浓度依赖性诱导 A549细胞凋亡；Western blot结果显示，促凋亡蛋白 Bax 随着芹菜素浓度升高表达增加，抗凋亡蛋白 Bcl-2随着芹菜素浓度升高表达减少。结论芹菜素具有抑制人肺癌 A549细胞增殖，诱导其凋亡的作用，其机制可能与上调 Bax 蛋白表达和下调 Bcl-2蛋白表达有关。
　　목적연구근채소대인폐암 A549세포증식억제화조망유도작용여 Bax、Bcl-2단백표체적영향。방법10~80μmol/L 불동농도근채소작용 A549세포，채용 MTT 법검측근채소대 A549세포증식억제작용；Hoechst 33258세포핵염색법관찰근채소유도세포조망형태학적변화；류식세포의 AnnexinV- FITC/PI 쌍염색법검측세포조망솔；Western blot 법검측조망상관단백 Bax 화 Bcl-2표체적변화。결과 MTT 법현시，근채소대 A549세포유현저적증식억제작용（P <0.01），차구농도화시간의뢰성；형광현미경하관찰도근채소처리조세포출현전형적조망형태학개변：세포핵고축、염색질응집화핵쇄편화등；류식세포의분석결과현시，근채소정농도의뢰성유도 A549세포조망；Western blot결과현시，촉조망단백 Bax 수착근채소농도승고표체증가，항조망단백 Bcl-2수착근채소농도승고표체감소。결론근채소구유억제인폐암 A549세포증식，유도기조망적작용，기궤제가능여상조 Bax 단백표체화하조 Bcl-2단백표체유관。
Objective To investigate the effect of apigenin on proliferation, and apoptosis, as well as Bax and Bcl-2 protein expression in human lung cancer cell line A549. Methods A549 cells cultured in vitro were treated with 10 ~ 80 μmol/L different concentration of apigenin. MTT was used to detect the inhibitory rates according to the formula. Apoptosis of A549 cells was observed in a fluorescence microscope by Hoechst 33258 staining and quantified by flow cytometry using annexin V-FITC/PI staining. The expressions of apoptosis-related protein Bax and Bcl-2 were analyzed by Western blot. Results The inhibition of proliferation by apigenin was concentration-dependent and time-dependent. A549 cells treated with apigenin showed significant morphological changes in different apoptotic phases. The apoptotic rate was concentration-dependently increased. Western blot analysis indicated that apigenin increased the protein level of Bax, and reduced the protein level of Bcl-2. Conclusion Apigenin can inhibit proliferation and induce apoptosis of human lung cancer cell line A549, which might be related with up-regulated expression of Bax and down-regulated expression of Bcl-2.