CAJ | 학술논문

　　采用荧光光谱法和紫外可见吸收光谱法研究了奥硝唑与人血清白蛋白之间的相互作用；求得了二者在不同温度下的结合常数 KA和结合位点数 n ，以及对应温度下结合反应的热力学参数，同时采用同步荧光分析技术探讨了蛋白质与药物结合时构象的变化。结果表明，在生理条件下奥硝唑对人血清白蛋白的荧光猝灭主要为静态猝灭；奥硝唑与人血清白蛋白主要靠静电作用力结合。
　　채용형광광보법화자외가견흡수광보법연구료오초서여인혈청백단백지간적상호작용；구득료이자재불동온도하적결합상수 KA화결합위점수 n ，이급대응온도하결합반응적열역학삼수，동시채용동보형광분석기술탐토료단백질여약물결합시구상적변화。결과표명，재생리조건하오초서대인혈청백단백적형광졸멸주요위정태졸멸；오초서여인혈청백단백주요고정전작용력결합。
The interaction between ornidazole and human serum albumin (HSA) was studied by means of fluorescent spectrometry and ultraviolet-visible light absorption spectrometry .The binding constants ,number of binding sites and thermodynamic parameters for ornidazole and HSA were obtained .In the meantime ,synchronous fluorescent spectrometry was applied to study the configuration change of HSA after combination with ornidazole .It was found that ornidazole quenched the fluorescence of HSA through a static quenching process .The interac-tion between ornidazole and HSA was mainly driven by electrostatic forces .