CAJ | 학술논문

　　利用紫外-可见吸收光谱、荧光光谱、圆二色谱等光谱手段，以及黏度测定等流体力学方法在三羟甲基氨基甲烷（tris）缓冲溶液和磷酸缓冲溶液中研究了含季铵盐联吡啶配体的钌（Ⅱ）配合物[Ru（L）（phen）2]（PF6）4（L ＝5，5′-二（三甲铵基甲基）-2，2′-联吡啶离子，phen ＝邻菲咯啉）与小牛胸腺DNA （CT-DNA ）的相互作用。结果表明，合成的配合物与CT-DNA之间存在一定的亲和作用，拟合得到的结合常数可达105；与此同时，该配合物能够稳定CT-DNA的结构，并对CT-DNA有较好的立体选择性（右旋异构体优先与DNA结合）。
　　이용자외-가견흡수광보、형광광보、원이색보등광보수단，이급점도측정등류체역학방법재삼간갑기안기갑완（tris）완충용액화린산완충용액중연구료함계안염련필정배체적조（Ⅱ）배합물[Ru（L）（phen）2]（PF6）4（L ＝5，5′-이（삼갑안기갑기）-2，2′-련필정리자，phen ＝린비각람）여소우흉선DNA （CT-DNA ）적상호작용。결과표명，합성적배합물여CT-DNA지간존재일정적친화작용，의합득도적결합상수가체105；여차동시，해배합물능구은정CT-DNA적결구，병대CT-DNA유교호적입체선택성（우선이구체우선여DNA결합）。
The interaction between culf thymus DNA (CT-DNA) and Ru(Ⅱ) complex [Ru(L) (phen)2 ](PF6 )4 (L = 5 ,5′-di(trimethylammoniomethyl)-2 ,2′-dipyridyl cation ,phen = 1 ,10-phenanthroline) was studied by means of ultraviolet-visible light absorption spectrometry ,flu-orescence spectrometry ,and circular dichroism spectrometry as well as viscosity measurements in tris(hydroxymethyl)aminomethane (denoted as tris) buffer and phosphoric acid buffer .Re-sults show that there exist a certain degree of affinity between as-synthesized Ru(Ⅱ) complex and CT-DNA ,and the intrinsic binding constant obtained by fitting is as much as 105 .In the meantime ,the Ru (Ⅱ ) complex can induce the stabilization of CT-DNA and possesses good steric selectivity towards CT-DNA (its right-handed enantiomer is preferentially bound to CT-DNA .