CAJ | 학술논문

　　采用电化学沉积法将铁氰化铈（CeHCF）薄膜修饰于玻碳电极（GCE）表面，得到铁氰化铈薄膜修饰玻碳电极；将血红蛋白（Hb）固载于该修饰电极表面，成功制得了 Hb/CeHCF/GCE过氧化氢生物传感器。考察了铁氰化铈薄膜修饰玻碳电极的氧化还原机理和制备条件，并对血红蛋白在电极上的电子传递过程进行了较为深入的研究。结果表明，铁氰化铈薄膜为血红蛋白提供了温和的固载环境，可实现血红蛋白与电极表面的直接电子转移，提高了血红蛋白的电化学活性；所制得的传感器对过氧化氢具有较高的催化响应和较强的稳定性。相关研究结果在生物医学和临床医学领域具有一定的借鉴意义。
　　채용전화학침적법장철청화시（CeHCF）박막수식우파탄전겁（GCE）표면，득도철청화시박막수식파탄전겁；장혈홍단백（Hb）고재우해수식전겁표면，성공제득료 Hb/CeHCF/GCE과양화경생물전감기。고찰료철청화시박막수식파탄전겁적양화환원궤리화제비조건，병대혈홍단백재전겁상적전자전체과정진행료교위심입적연구。결과표명，철청화시박막위혈홍단백제공료온화적고재배경，가실현혈홍단백여전겁표면적직접전자전이，제고료혈홍단백적전화학활성；소제득적전감기대과양화경구유교고적최화향응화교강적은정성。상관연구결과재생물의학화림상의학영역구유일정적차감의의。
Cerium ferricyanide film (CeHCF) was electrochemically deposited onto the surface of glassy carbon electrode (GCE) to afford GCE modified by CeHCF .Onto the surface of CeH-CF-modified GCE was then immobilized hemoglobin (Hb) to provide Hb/CeHCF/GCE as a hydrogen peroxide biosensor . The oxidation-reduction mechanisms of the CeHCF-modified GCE and the experimental conditions for preparing this modified GCE were examined ;and the electron transfer process of Hb on the surface of the modified GCE was investigated .Results show that cerium ferricyanide film provides a gentle immobility environment for hemoglobin thereby realizing the direct electron transfer between Hb and the electrode as well as improving the electrochemical activity of Hb .As-fabricated Hb/CeHCF/GCE exhibits good catalytic re-sponse to hydrogen peroxide and has good stability as well .Relevant research results may be of significance in the fields of biomedicine and clinical inspection .