激光技术
激光技術
격광기술
LASER TECHNOLOGY
2013年
4期
487-492
,共6页
散射%图谱分布%反常衍射近似%红细胞%扁椭球模型%数值分析%实验验证
散射%圖譜分佈%反常衍射近似%紅細胞%扁橢毬模型%數值分析%實驗驗證
산사%도보분포%반상연사근사%홍세포%편타구모형%수치분석%실험험증
scattering%spectrum distribution%anomalous diffraction approximation%red blood cell%oblate ellipsoid model%numerical analysis%experiment proving
为了获取流体中血红细胞精确的光散射信息,以反常衍射近似理论为基础,采用扁椭球构建了流体中血红细胞的数学散射模型。应用数值仿真模拟技术,分别讨论了血红细胞形体变化、体积变化、取向角和相对折射率变化对光散射幅值分布的影响,建立了相应的图谱分布,并通过实验对此模型进行了验证。结果表明,在前向小角度内,不同血红细胞大小、不同相对折射率对光散射信息有着较大影响。这一结果对于病理血红细胞的快速准确检测和流式细胞仪等细胞测量仪的精确测量是有帮助的。
為瞭穫取流體中血紅細胞精確的光散射信息,以反常衍射近似理論為基礎,採用扁橢毬構建瞭流體中血紅細胞的數學散射模型。應用數值倣真模擬技術,分彆討論瞭血紅細胞形體變化、體積變化、取嚮角和相對摺射率變化對光散射幅值分佈的影響,建立瞭相應的圖譜分佈,併通過實驗對此模型進行瞭驗證。結果錶明,在前嚮小角度內,不同血紅細胞大小、不同相對摺射率對光散射信息有著較大影響。這一結果對于病理血紅細胞的快速準確檢測和流式細胞儀等細胞測量儀的精確測量是有幫助的。
위료획취류체중혈홍세포정학적광산사신식,이반상연사근사이론위기출,채용편타구구건료류체중혈홍세포적수학산사모형。응용수치방진모의기술,분별토론료혈홍세포형체변화、체적변화、취향각화상대절사솔변화대광산사폭치분포적영향,건립료상응적도보분포,병통과실험대차모형진행료험증。결과표명,재전향소각도내,불동혈홍세포대소、불동상대절사솔대광산사신식유착교대영향。저일결과대우병리혈홍세포적쾌속준학검측화류식세포의등세포측량의적정학측량시유방조적。
In order to obtain accurate light scattering information of red blood cell in the fluid flow , the scattering model of red blood cell in the fluid flow was constructed based on the anomalous diffraction approximation and the oblate ellipsoid.The effect of the variety of body, volume, relative refractive index and orientation angle on the scattering amplitude was studied.The simulation data indicate that the volume and relative refractive index of different red blood cell has the prominent effect on the scattering information at small orientation angles .The model was proved by experiments . This result is helpful for measuring red blood cell fast and accurately and the precise measurement of the cell measurement instrument such as flow cytometer.