CAJ | 학술논문

　　目的：建立一种实用、可靠的大鼠脊髓损伤(SCI)模型，为进一步研究SCI提供实验基础。方法：30只雄性Wistar大鼠随机分为SCI模型组、假手术组和正常对照组，每组10只。采用自制击打器击打T10对应的脊髓节段建立SCI模型。分别于术前1d，术后1d、7d、14d、21d和28d对大鼠进行Basso-Beattie-Bresnahan(BBB)评分，观察大鼠SCI模型的建立情况。结果：大鼠麻醉清醒后，假手术组大鼠行走轻度异常，术后1d完全恢复正常；SCI模型组大鼠损伤平面以下完全瘫痪。术后第1d、7d、14d、21d、28d，SCI模型组大鼠的BBB评分均明显低于假手术组、正常组同时间点(P＜0.05)。结论：本研究建立大鼠SCI模型的方法可靠，自制击打器操作简便，可用于SCI的相关研究。
　　목적：건립일충실용、가고적대서척수손상(SCI)모형，위진일보연구SCI제공실험기출。방법：30지웅성Wistar대서수궤분위SCI모형조、가수술조화정상대조조，매조10지。채용자제격타기격타T10대응적척수절단건립SCI모형。분별우술전1d，술후1d、7d、14d、21d화28d대대서진행Basso-Beattie-Bresnahan(BBB)평분，관찰대서SCI모형적건립정황。결과：대서마취청성후，가수술조대서행주경도이상，술후1d완전회복정상；SCI모형조대서손상평면이하완전탄탄。술후제1d、7d、14d、21d、28d，SCI모형조대서적BBB평분균명현저우가수술조、정상조동시간점(P＜0.05)。결론：본연구건립대서SCI모형적방법가고，자제격타기조작간편，가용우SCI적상관연구。
Objective:Toestablishausefulandreliablespinalcordinjury(SCI)rats’modelandprovideexperimental basisforfurtherresearchofthemechanismsofSCI.Methods:30maleWistarratswererandomlydividedinto3groups with10ratsineachgroup:SCImodelgroup,sham-operationgroupandnormalcontrolgroup.TheSCIrats’modelwas established by beating the T10 corresponding spinal segment using special equipment. The Basso-Beattie-Bresnahan locomotortatingscale(BBBscores)wasevaluated1dbeforeoperation,1d,7d,14d,21dand28dafteroperationrespectively. Results:Afteroperation,theratsinsham-operationgroupwalkedslightlyabnormalandcompletelyrecoveredin1day;The ratsinSCImodelgroupcompletelyparalyzedbelowtheinjurylevel.TheBBBscoresofratsinSCImodelgroup1d,7d,14d, 21dand28dafteroperationwerealllowerthanthatofratsinsham-operationgroupandnormalcontrolgroupatthesame timepoint(P<0.05). Conclusions:TheSCIestablishingmethodinthisstudyisreliableandcanbeusedinSCIresearch, andthespecialequipmentinthisstudyiseasytooperate.