CAJ | 학술논문

　　目的：建立相同产地黄芩药材的HPLC指纹图谱，以探讨相同产地黄芩药材的个体差异。方法：采用HPLC法测定20个采自内蒙古赤峰的黄芩样品的HPLC指纹图谱，色谱条件：Agilent TC-C18柱(4.6mm×250mm，5μm)，乙腈与0.1%冰醋酸溶液进行梯度洗脱，检测波长276nm，流速1ml/min，柱温25℃。运用中药色谱指纹图谱相似度评价系统分析各个样品的相似度。结果：20个黄芩药材样品的HPLC指纹图谱主要有6个共有峰，样品相似度均达到0.9以上，但共有峰相对面积有一定的差异。结论：相同产地黄芩HPLC指纹图谱分析方法可靠，可为以后研究不同产地黄芩药材HPLC指纹图谱奠定基础。
　　목적：건립상동산지황금약재적HPLC지문도보，이탐토상동산지황금약재적개체차이。방법：채용HPLC법측정20개채자내몽고적봉적황금양품적HPLC지문도보，색보조건：Agilent TC-C18주(4.6mm×250mm，5μm)，을정여0.1%빙작산용액진행제도세탈，검측파장276nm，류속1ml/min，주온25℃。운용중약색보지문도보상사도평개계통분석각개양품적상사도。결과：20개황금약재양품적HPLC지문도보주요유6개공유봉，양품상사도균체도0.9이상，단공유봉상대면적유일정적차이。결론：상동산지황금HPLC지문도보분석방법가고，가위이후연구불동산지황금약재HPLC지문도보전정기출。
Objective:TostudythedifferenceofScutellariabaicalensisfromsameproductionplacesbyestablishing HPLC fingerprints of Scutellaria baicalensis. Methods:HPLC fingerprints of 20 Scutellaria baicalensis samples from NeimengguChifengwereanalyzedbyHPLC.Thechromatographiccondition:AgilentTC–C18Column(4.6mm×250mm, 5μm),mobilephasewasacetonitrileand0.1%glacialaceticacidingradientelutionprogram,detectingwavelength276nm, flow rate 1.0 ml/min, and column temperature 25℃. The similarity of each sample was analyzed by chromatographic ifngerprint similarity evaluation system. Results:20 Scutellaria baicalensis samples had 6 common peaks. The similarity of 20 samples were all higher than 0.9, but there had some differences between the relative area of the common peaks. Conclusions:HPLCifngerprintsofScutellariabaicalensisfromsameproductionplaceswillprovideareferencestandardfor thestudyofScutellariabaicalensisfromdifferentproductionplaces.