中国有色金属学报（英文版）
中國有色金屬學報（英文版）
중국유색금속학보（영문판）
TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA
2013年
7期
2153-2159
,共7页
Acidiphilium cryptum%生物浸出%黄铁矿%磷矿%生物溶解%Acidithiobacillus ferrooxidans
Acidiphilium cryptum%生物浸齣%黃鐵礦%燐礦%生物溶解%Acidithiobacillus ferrooxidans
Acidiphilium cryptum%생물침출%황철광%린광%생물용해%Acidithiobacillus ferrooxidans
Acidiphilium cryptum%bioleaching%pyrite%rock phosphate%biosolubilization%Acidithiobacillus ferrooxidans
比较了嗜酸自养菌Acidithiobacillus ferrooxidans、嗜酸异养菌Acidiphilium cryptum、At. ferrooxidans和A. cryptum的混合菌在9K基体盐培养基中对黄铁矿的生物浸出以及磷矿的生物溶解。结果表明，虽然A. cryptum自身不能氧化黄铁矿和溶解磷矿，但能有效促进 At. ferrooxidans对黄铁矿的生物浸出以及磷矿的生物溶解。这种促进效应可通过A. cryptum促进At. ferrooxidans生物浸出黄铁矿体系中pH的降低以及氧化还原电位、总铁浓度和浮游细菌数目的升高的实验结果来证明。At. ferrooxidans和A. cryptum的混合菌液在30°C条件下溶解磷矿时可最大程度地释放其中的可溶性磷。矿浆浓度大于3%时会给At. ferrooxidans和A. cryptum的混合菌液释放可溶性磷带来不利影响。在9K基体盐培养基中添加黄铁矿对At. ferrooxidans和A. cryptum的混合菌液溶解磷矿是很有必要的，且磷矿和黄铁矿的质量比为1：2或1：3时可溶性磷浸出率较高。
比較瞭嗜痠自養菌Acidithiobacillus ferrooxidans、嗜痠異養菌Acidiphilium cryptum、At. ferrooxidans和A. cryptum的混閤菌在9K基體鹽培養基中對黃鐵礦的生物浸齣以及燐礦的生物溶解。結果錶明，雖然A. cryptum自身不能氧化黃鐵礦和溶解燐礦，但能有效促進 At. ferrooxidans對黃鐵礦的生物浸齣以及燐礦的生物溶解。這種促進效應可通過A. cryptum促進At. ferrooxidans生物浸齣黃鐵礦體繫中pH的降低以及氧化還原電位、總鐵濃度和浮遊細菌數目的升高的實驗結果來證明。At. ferrooxidans和A. cryptum的混閤菌液在30°C條件下溶解燐礦時可最大程度地釋放其中的可溶性燐。礦漿濃度大于3%時會給At. ferrooxidans和A. cryptum的混閤菌液釋放可溶性燐帶來不利影響。在9K基體鹽培養基中添加黃鐵礦對At. ferrooxidans和A. cryptum的混閤菌液溶解燐礦是很有必要的，且燐礦和黃鐵礦的質量比為1：2或1：3時可溶性燐浸齣率較高。
비교료기산자양균Acidithiobacillus ferrooxidans、기산이양균Acidiphilium cryptum、At. ferrooxidans화A. cryptum적혼합균재9K기체염배양기중대황철광적생물침출이급린광적생물용해。결과표명，수연A. cryptum자신불능양화황철광화용해린광，단능유효촉진 At. ferrooxidans대황철광적생물침출이급린광적생물용해。저충촉진효응가통과A. cryptum촉진At. ferrooxidans생물침출황철광체계중pH적강저이급양화환원전위、총철농도화부유세균수목적승고적실험결과래증명。At. ferrooxidans화A. cryptum적혼합균액재30°C조건하용해린광시가최대정도지석방기중적가용성린。광장농도대우3%시회급At. ferrooxidans화A. cryptum적혼합균액석방가용성린대래불리영향。재9K기체염배양기중첨가황철광대At. ferrooxidans화A. cryptum적혼합균액용해린광시흔유필요적，차린광화황철광적질량비위1：2혹1：3시가용성린침출솔교고。
The bioleaching of pyrite and biosolubilization of rock phosphate (RP) in 9K basal salts medium were compared by the following strains of an autotrophic acidophilic bacterium, Acidithiobacillus ferrooxidans, a heterotrophic acidophilic bacterium, Acidiphilium cryptum, and mixed culture of At. ferrooxidans and A. cryptum. The results show that A. cryptum is effective in enhancing the bioleaching of pyrite and biosolubilization of RP in the presence of At. ferrooxidans, although it could not oxidize pyrite and solubilize RP by itself. This effect is demonstrated experimentally that A. cryptum enhances a decrease in pH and an increase in redox potential, concentration of total soluble iron and planktonic part bacterial number in the broth during pyrite bioleaching processes by At. ferrooxidans. The mixed culture of At. ferrooxidans and A. cryptum leads to the most extensive soluble phosphate released at 30 °C. Pulp density exceeding 3% is shown to adversely influence the release of soluble phosphate by the consortium of At. ferrooxidans and A. cryptum. It is essential to add pyrite to the 9K basal salts medium for the biosolubilization of RP by the mixed culture of At. ferrooxidans and A. cryptum, and the percentage of soluble phosphate released is the greatest when the mass ratio of RP to pyrite is 1:2 or 1:3.
