CAJ | 학술논문

　　采用酰胺化反应方法，通过Fe3O4纳米颗粒表面氨基基团和与肿瘤细胞具有高度亲和力的叶酸分子的羧基间反应进行偶联得到目标材料，利用X射线衍射、傅立叶红外光谱仪、透射电镜、振动样品磁强计、动态光散射仪等对所合成的材料进行表征，且以瘤鼠为动物模型，考察其活体肝癌细胞标记效果和MRI成像功能。结果表明，合成的材料为立方相的Fe3O4，粒径约8 nm，水合直径约25.7 nm，呈近似球形形貌，表面分布有羧基等功能基团，呈超顺磁特性，饱和磁化强度为51 A·m2/kg，能靶向进入肝癌细胞内，提高肿瘤与周围正常组织的MRI成像信号对比。实验证实，叶酸偶联的超顺磁Fe3O4纳米颗粒，不仅细胞毒性小，而且因其表面的叶酸与叶酸受体之间的高强结合力，能通过配体?受体结合作用被高效介导进入肿瘤细胞内，实现对癌细胞的标记、示踪、MRI靶向检测及磁热疗。
　　채용선알화반응방법，통과Fe3O4납미과립표면안기기단화여종류세포구유고도친화력적협산분자적최기간반응진행우련득도목표재료，이용X사선연사、부립협홍외광보의、투사전경、진동양품자강계、동태광산사의등대소합성적재료진행표정，차이류서위동물모형，고찰기활체간암세포표기효과화MRI성상공능。결과표명，합성적재료위립방상적Fe3O4，립경약8 nm，수합직경약25.7 nm，정근사구형형모，표면분포유최기등공능기단，정초순자특성，포화자화강도위51 A·m2/kg，능파향진입간암세포내，제고종류여주위정상조직적MRI성상신호대비。실험증실，협산우련적초순자Fe3O4납미과립，불부세포독성소，이차인기표면적협산여협산수체지간적고강결합력，능통과배체?수체결합작용피고효개도진입종류세포내，실현대암세포적표기、시종、MRI파향검측급자열료。
Highly biocompatible superparamagnetic Fe3O4 nanoparticles were synthesized by amide of folic acid (FA) ligands and the NH2-group onto the surface of Fe3O4 nanoparticles. The as-synthesized folate-conjugated Fe3O4 nanoparticles were characterized by X-ray diffraction diffractometer, transmission electron microscope, FT-IR spectrometer, vibrating sample magnetometer, and dynamic light scattering instrument. The in vivo labeling effect of folate-conjugated Fe3O4 nanoparticles on the hepatoma cells was investigated in tumor-bearing rat. The results demonstrate that the as-prepared nanoparticles have cubic structure of Fe3O4 with a particle size of about 8 nm and hydrated diameter of 25.7 nm at a saturation magnetization of 51 A·m2/kg. These nanoparticles possess good physiological stability, low cytotoxicity on human skin fibroblasts and negligible effect on Wistar rats at the concentration as high as 3 mg/kg body mass. The folate-conjugated Fe3O4 nanoparticles could be effectively mediated into the human hepatoma Bel 7402 cells through the binding of folate and folic acid receptor, enhancing the signal contrast of tumor tissue and surrounding normal tissue in MRI imaging. It is in favor of the tumor cells labeling, tracing, magnetic resonance imaging (MRI) target detection and magnetic hyperthermia.