CAJ | 학술논문

目的：检测上皮性卵巢癌中基质金属蛋白酶-2（matrix metalloproteinase -2，MMP-2）及金属蛋白酶组织抑制剂-2（ tissue inhibitor of metalloproteinase -2，TIMP-2）的表达水平，并分析其临床意义。方法采用免疫组化染色法检测MMP-2、TIMP-2在上皮性卵巢癌、上皮性良性卵巢肿瘤及正常卵巢组织3组对象卵巢组织中的阳性表达率，分析上皮性卵巢癌患者不同临床病理特征下MMP-2及TIMP-2的阳性表达水平。结果上皮性卵巢癌组卵巢组织MMP-2及TIMP-2的阳性表达率明显高于良性上皮性卵巢肿瘤组和正常卵巢组织组（ P＜0.05）。 MMP-2及TIMP-2在上皮性卵巢癌Ⅲ期＋Ⅳ期患者中的阳性表达率明显高于Ⅰ期＋Ⅱ期患者（ P＜0.05）；在低分化（ G3）患者中的阳性表达率明显高于高、中分化（ G1＋G2）患者（P＜0.05）；在有淋巴结转移患者中的阳性表达率明显高于无淋巴结转移患者（P＜0.05）。结论 MMP-2和TIMP-2的检测是否可作为早期诊断上皮性卵巢癌的生物指标，尚需进一步研究，但MMP-2及TIMP-2的表达水平与上皮性卵巢癌的临床分期、分化程度、淋巴结转移有关，可作为判断上皮性卵巢癌预后的辅助指标。
목적：검측상피성란소암중기질금속단백매-2（matrix metalloproteinase -2，MMP-2）급금속단백매조직억제제-2（ tissue inhibitor of metalloproteinase -2，TIMP-2）적표체수평，병분석기림상의의。방법채용면역조화염색법검측MMP-2、TIMP-2재상피성란소암、상피성량성란소종류급정상란소조직3조대상란소조직중적양성표체솔，분석상피성란소암환자불동림상병리특정하MMP-2급TIMP-2적양성표체수평。결과상피성란소암조란소조직MMP-2급TIMP-2적양성표체솔명현고우량성상피성란소종류조화정상란소조직조（ P＜0.05）。 MMP-2급TIMP-2재상피성란소암Ⅲ기＋Ⅳ기환자중적양성표체솔명현고우Ⅰ기＋Ⅱ기환자（ P＜0.05）；재저분화（ G3）환자중적양성표체솔명현고우고、중분화（ G1＋G2）환자（P＜0.05）；재유림파결전이환자중적양성표체솔명현고우무림파결전이환자（P＜0.05）。결론 MMP-2화TIMP-2적검측시부가작위조기진단상피성란소암적생물지표，상수진일보연구，단MMP-2급TIMP-2적표체수평여상피성란소암적림상분기、분화정도、림파결전이유관，가작위판단상피성란소암예후적보조지표。
Objective To detect the expression level of matrix metalloproteninase -2 ( MMP -2 ) and its inhibitor tissue inhibitor of metalloprotenise -2( TIMP-2) in patients with epithelial ovarian cancer , and to analyze its clinical significance .Methods Immunohistochemical staining technique was used to detect the expression of MMP-2, TIMP-2 in 3 groups of cases with epithelial ovarian cancer , benign epithelial ovarian tumor and normal ovarian tissues separately .The positive expression rate of MMP -2 and TIMP-2 in patients with epithelial ovarian cancer of different pathological characters were analyzed .Results The positive expression rates of MMP -2 and TIMP-2 in epithelial ovarian cancer group were significantly higher than benign ovarian tumor group and normal ovarian tissues group ( P<0.05) .The positive expression rates of MMP -2 and TIMP-2 in patients with epithelial ovarian cancer stage III +IV were significantly higher than that in patients with stage I +II ( P <0.05 ) , The positive expression rates of MMP -2 and TIMP-2 in patients with low differentiation ( G3 ) were significantly higher than that in patients with high differentiation (G1 +G2) (P<0.05).The positive expression rates of MMP -2 and <br> TIMP-2 in patients with lymph node metastasis were significantly higher than that in patients without lymph node metastasis (P<0.05).Conclusion Further research is needed as to whether detection of MMP -2 and TIMP-2 can be used for early diagnosis of epithelial ovarian cancer ,but the expression levels of MMP -2 and TIMP-2 are related to staging , differentiation , lymph node metastasis in epithelial ovarian cancer , which can be used as auxiliary indicators of prognosis in epithelial ovarian carcinoma .