CAJ | 학술논문

背景：假体周围微量元素Zn的含量与骨形态发生蛋白7、Stro-1+细胞相关性可能影响髋关节假体的骨融合与生存率。 　　目的：分析股骨近端微量元素Zn与骨形态发生蛋白7、Stro-1细胞的相关性。 　　方法：24例初次髋关节置换患者，取术中股骨矩开槽时废弃骨块，提取细胞后体外培养骨髓间充质干细胞。培养14 d采用流式细胞仪检测骨髓间充质干细胞中的Stro-1+细胞，酶联免疫吸附测定试剂盒检测培养细胞离心上清液中骨形态发生蛋白7的表达，放射免疫荧光法检测培养细胞离心上清液中Zn含量。 　　结果与结论：Zn含量在不同年龄组及不同性别组差异无显著性意义；Zn与Stro-1+、骨形态发生蛋白7正相关联系。关于股骨近端微量元素Zn的进一步研究可以用来预测和干预髋关节假体的寿命。
배경：가체주위미량원소Zn적함량여골형태발생단백7、Stro-1+세포상관성가능영향관관절가체적골융합여생존솔。 　　목적：분석고골근단미량원소Zn여골형태발생단백7、Stro-1세포적상관성。 　　방법：24례초차관관절치환환자，취술중고골구개조시폐기골괴，제취세포후체외배양골수간충질간세포。배양14 d채용류식세포의검측골수간충질간세포중적Stro-1+세포，매련면역흡부측정시제합검측배양세포리심상청액중골형태발생단백7적표체，방사면역형광법검측배양세포리심상청액중Zn함량。 　　결과여결론：Zn함량재불동년령조급불동성별조차이무현저성의의；Zn여Stro-1+、골형태발생단백7정상관련계。관우고골근단미량원소Zn적진일보연구가이용래예측화간예관관절가체적수명。
BACKGROUND:The correlation of zinc with Stro-1+cells and bone morphogenetic protein 7 surrounding the prosthesis may affect the bone fusion and survival rate in hip prosthesis. OBJECTIVE:To analyze the correlation of zinc content with Stro-1+cells and bone morphogenetic protein 7 in proximal femur. METHODS:Bone samples were obtained from the discarded metaphysis region of the proximal femur in 24 patients with primary total hip replacement. Bone marrow mesenchymal stem cells were cultured in vitro. At 14 days after culture, Stro-1+cells in bone marrow mesenchymal stem cells were detected using flow cytometry. Bone morphogenetic protein 7 expression in cellsupernatant was detected using enzyme-linked immunosorbent assay. Zinc content in supernatant was measured using radio-immunity assay. RESULTS AND CONCLUSION:No significant difference in the zinc content was detected in different age groups and different gender groups. Zinc content was positively associated with Stro-1+cells and bone morphogenetic protein 7 expression. The further study of the trace element zinc in proximal femur can predict and intervene the longevity of hip prosthesis.