农产品加工·学刊(下)
農產品加工·學刊(下)
농산품가공·학간(하)
Academic Periodical of Farm Products Processing
2013年
7期
1-3,8
,共4页
保加利亚乳杆菌%培养基%优化
保加利亞乳桿菌%培養基%優化
보가리아유간균%배양기%우화
Lactobacillus buglaricus%medium%optimization
对具有黏度高和后酸化度低的特性的一株德氏乳杆菌菌保加利亚亚种(Lactobacillus bulgaricus) LB5的最佳培养基进行研究,根据保加利亚乳杆菌的营养需求,以MRS为基础培养基,先确定最佳的碳源、氮源、缓冲盐等,再通过响应面对主要因子进行筛选和优化,最终确定LB5的培养基为:葡萄糖16.50 g/L,大豆蛋白胨24.00 g/L,酪蛋白胨5.00 g/L,酵母浸粉16.00 g/L,柠檬酸3.30 g/L,柠檬酸钠4.08 g/L。
對具有黏度高和後痠化度低的特性的一株德氏乳桿菌菌保加利亞亞種(Lactobacillus bulgaricus) LB5的最佳培養基進行研究,根據保加利亞乳桿菌的營養需求,以MRS為基礎培養基,先確定最佳的碳源、氮源、緩遲鹽等,再通過響應麵對主要因子進行篩選和優化,最終確定LB5的培養基為:葡萄糖16.50 g/L,大豆蛋白胨24.00 g/L,酪蛋白胨5.00 g/L,酵母浸粉16.00 g/L,檸檬痠3.30 g/L,檸檬痠鈉4.08 g/L。
대구유점도고화후산화도저적특성적일주덕씨유간균균보가리아아충(Lactobacillus bulgaricus) LB5적최가배양기진행연구,근거보가리아유간균적영양수구,이MRS위기출배양기,선학정최가적탄원、담원、완충염등,재통과향응면대주요인자진행사선화우화,최종학정LB5적배양기위:포도당16.50 g/L,대두단백동24.00 g/L,락단백동5.00 g/L,효모침분16.00 g/L,저몽산3.30 g/L,저몽산납4.08 g/L。
The aim of the test is to design optimal medium for LB5 which is Lactobacillus buglaricus (L.b) . LB5 has charac-teristics of high viscosity and low acidification. According to its nutrition of requirement and at the basis of MRS, all ingredi-ents comprise of carbon source, nitrogen source and buffer salts which are adjusted in order to form the optimum medium for LB5. Lastly, major factors are optimized by response surface, optimal medium is determined by the Multi-Regression Equa-tion as follows glucose 16.50 g/L, soy peptone 24.00 g/L, extract of yeast 16.00 g/L, casein peptone 5.00 g/L, citric acid 3.30 g/L and sodium citrate 4.08 g/L.