CAJ | 학술논문

　　以暗培养7 d的蓖麻上胚轴为外植体，通过研究不同浓度TDZ对上胚轴不定芽诱导的影响，以及BA和GA3不同配比对不定芽伸长的影响，建立高效的蓖麻体外繁殖体系。芽诱导阶段，上胚轴接种在添加了TDZ的MS （Murashige and Skoog）培养基上，结果表明， TDZ质量浓度为0.14 mg/L时，诱导的不定芽个数最多（24.07），同时出芽率也较好（80.61%）； TDZ质量浓度为0.11 mg/L时，不定芽个数为20.06，出芽率最高（82.38%）。根据诱导试验表明，添加活性炭（AC）和0.30 mg/L的1/2 MS培养基，根诱导效果最好，达95%以上。移栽后，植株成活率为80%。
　　이암배양7 d적비마상배축위외식체，통과연구불동농도TDZ대상배축불정아유도적영향，이급BA화GA3불동배비대불정아신장적영향，건립고효적비마체외번식체계。아유도계단，상배축접충재첨가료TDZ적MS （Murashige and Skoog）배양기상，결과표명， TDZ질량농도위0.14 mg/L시，유도적불정아개수최다（24.07），동시출아솔야교호（80.61%）； TDZ질량농도위0.11 mg/L시，불정아개수위20.06，출아솔최고（82.38%）。근거유도시험표명，첨가활성탄（AC）화0.30 mg/L적1/2 MS배양기，근유도효과최호，체95%이상。이재후，식주성활솔위80%。
In this present study, the effect of all concentrations of TDZ (Thidiazuron) on shoot induction and the effect of combination of BA and GA3 on shoot elongation are studied. As a result, an efficient protocol of castor tissue culture with epicotyl explant is established. Epicotyl is incubated on MS (Murashige and Skoog) medium. For shoot induction, 0.14 mg/L TDZ gives the maximum number of shoots (24.07) with the second best frequency of explants producing shoots (80.61%);while 0.11 mg/L TDZ with a high number of shoots (20.06) is found best for the frequency of explants producing shoots (82.38%) . Strong healthy shoots ere incubated on half strength of MS medium supplemented with 0.30 mg/L IBA and 1%AC with 95%root induction rate. About 80%of the plantlets ere survived after transplanting into soil.