CAJ | 학술논문

　　蛹虫草中含有多种生理活性物质，是中国名贵中药材之一。前期试验发现其发酵液中含有纤溶酶，具有开发成一种新型溶栓药物的价值。以试验室保存的8株蛹虫草为试验菌株，以纤溶酶酶活为指标，进行液体深层培养，确定以C-5作为诱变出发菌株，对其进行原生质体紫外诱变处理，筛选得到31株抗制霉素突变株，抗性菌株以纤溶酶酶活为指标进行液体深层发酵，得到8株纤溶酶产量高于出发菌株15.00%以上的突变株，并从中筛选出3株遗传稳定性良好的正突变株CY-8， CY-18， CY-25，其纤溶酶产量分别比出发菌株提高了28.58%，28.22%，21.78%。
　　용충초중함유다충생리활성물질，시중국명귀중약재지일。전기시험발현기발효액중함유섬용매，구유개발성일충신형용전약물적개치。이시험실보존적8주용충초위시험균주，이섬용매매활위지표，진행액체심층배양，학정이C-5작위유변출발균주，대기진행원생질체자외유변처리，사선득도31주항제매소돌변주，항성균주이섬용매매활위지표진행액체심층발효，득도8주섬용매산량고우출발균주15.00%이상적돌변주，병종중사선출3주유전은정성량호적정돌변주CY-8， CY-18， CY-25，기섬용매산량분별비출발균주제고료28.58%，28.22%，21.78%。
The fungus Cordyceps militaris one of the famous Chinese traditional medicinal mushroom and fibrinolytic enzyme is contained in its fermentation liquid, which is expected to be a new thrombolytic drug. To obtain the highest activity of fibrinolytic enzyme, the submerged liquid fermentation of eight Cordycps militaris strains from our lab are studied. C-1 as original strain, its protoplast is mutated, and 31 nystatin-resistant mutants are obtained. All mutants are second screened through repeated submerged, 8 fibrinolytic enzyme higher 15.00% of mutant and then 3 positive mutants with highly genetic stability, CY-8, CY-18 and CY-25 are obtained. The productivity of the fibrinolytic enzyme of positive mutants increased by 28.58%, 28.22%and 21.78%respectively compared with that of original strain.